DEHYDRATED CULTURE MEDIA 89 



inocula producing colonies that were readily recognized. These media were easily 

 prepared and in addition were economical. They were also satisfactorily em- 

 ployed in streptomycin sensitivity tests. 



To rehydrate the medium, suspend 40 grams of Bacto-Blood Agar Base in 

 1000 ml. of col J distilled water and heat to boiling to dissolve the medium com- 

 pletely. Distribute in tubes or flasks and sterilize in the autoclave for 15 minutes 

 at 15 pounds pressure (121°C.). The final reaction of the medium, before add- 

 ing blood, will be pH 6.8. 



If Blood Agar is to be prepared immediately, the sterile medium is cooled at 

 once to 45-50° C, and, while still liquid, 5 per cent sterile defibrinated blood is 

 added aseptically with thorough mixing, avoiding incorporation of air bubbles, 

 and distributing in sterile tubes or plates as desired. Blood Agar should be 

 incubated before use to insure sterility. 



Since most microorganisms prefer a fresh medium wdth a moist surface it is 

 recommended that Blood Agar Base be prepared as required, or melted and re- 

 solidified just prior to use. Blood Agar from this medium should be prepared as 

 required. 



One pound of Bacto-Blood Agar Base will make 11.3 liters of the finished 

 medium. 



IJ. Lab. Clin. Med., 17:558:1932. Edition: 70: 1950. 



?Di«s>iostic Procedures and Reagents, 3rd "Am. J, Clin. Path., 21:101:1951. 



BACTO 



VEAL INFUSION AGAR (B343) 



DEHYDRATED 



Veal Heart, Infusion from 500 g. 



Proteose Peptone, No. 3, Difco .... 10 g. 



Sodium Chloride 5 g. 



Bacto-Agar 15 g. 



Bacto-Veal Infusion Agar is prepared from select lean veal and is recom- 

 mended as a solid medium for the cultivation of fastidious pathogenic bacteria. 

 It is also a suitable basal medium for enrichment by the addition of blood, 

 ascitic fluid, serum or other enrichments. 



To rehydrate the medium suspend 40 grams Bacto-Veal Infusion Agar in 

 1000 ml. cold distilled water and heat to boiling to dissolve the medium com- 

 pletely. Distribute in tubes or flasks and sterilize in the autoclave for 15 minutes 

 at 15 pounds pressure (121°G.). 



Since most organisms prefer a fresh medium with a moist surface, it is sug- 

 gested that the medium be prepared as required or melted and re-solidified just 

 prior to use. The final reaction of the medium will be pH 7.4. 



One pound of Bacto-Veal Infusion Agar will make 11.3 liters of medium. 



BACTO 



PPLO AGAR (B412) 



DEHYDRATED 



Bacto-Beef Heart for Infusions, 



Infusion from 50 g. 



Bacto- Peptone 10 g. 



Sodium Chloride 5 g. 



Bacto-Agar 14 g. 



