94 DIFCOMANUAL 



period specified. The flasks can be used to pour plates at once and tubes may be 

 melted in boiling water and used as needed. 



One pound of Bacto-Mueller Hinton Medium is sufficient to prepare 11.9 

 liters of medium. 



1 Proc. Soc. Exp. Biol. Med., 48:330:1941. * Personal Communication. 



2 Brit. Med. J., 2:678:1916. ^ Diagnostic Procedures and Reagents, 



3 J. Am. Med. Assoc, 123:547:1943. 3rd Edition: 192: 1950. 



BACTO 



BRAIN VEAL AGAR (B49) 



DEHYDRATED 



Calf Brain, Infusion from 250 g. 



Veal, Infusion from 375 g. 



Proteose Peptone, Difco 10 g, 



Monosodium Phosphate 1.25 g. 



Sodium Chloride 3.75 g. 



Bacto-Agar 15 g. 



Bacto-Brain Veal Agar is a medium containing extractives of fresh calf brain 

 and veal. It was developed primarily for the cultivation of the gonococcus. This 

 medium also supports good growth of streptococci, pneumococci, meningococci, 

 and other microorganisms generally considered difficult to cultivate. 



Pelouze and Viteri,^ following a study of the cultural requirements of the 

 gonococcus, devised a simple medium, Brain Veal Agar, upon which this diplo- 

 coccus grew luxuriantly. Bacto-Brain Veal Agar is prepared according to their 

 formula and gives results comparable to the medium prepared by them. In 

 addition, it is an excellent medium for other highly fastidious microorganisms. 

 Bacto-Brain Veal Agar is a satisfactory medium for the preparation of bacterial 

 antigens and vaccines. 



For the cultivation of the gonococcus for complement-fixation work Garcia^ 

 reported that Bacto-Brain Veal Agar gave good growth. Saccone^ used Bacto- 

 Brain Veal Agar for the isolation of the gonococcus. Reitzel and Kohl,* in their 

 modification of the McLeod method for the isolation of the gonocccus, employed 

 a medium more adaptable for use in small laboratories. Their medium was pre- 

 pared by using a combination of Bacto-Brain Heart Infusion as discussed on 

 page 77 and Bacto-Brain Veal Agar which, when mixed with an equal quantity 

 of Bacto-Hemoglobin as discussed on page 271, would give a final concentration 

 of 1.2 per cent agar. Shaw^ added 0.5 per cent cystine and 1 per cent dextrose 

 to Bacto-Brain Veal Agar for the cultivation of Pasteurella tularensis with ex- 

 cellent results. Foshay^ also used Bacto-Brain Veal Agar with Nutrose (sodium 

 caseinate), dextrose, cystine, inorganic salts and serous enrichments for the 

 propagation of P. tularensis. 



Although Bacto-Brain Veal Agar will support good growth of the gonococcus, 

 media prepared without infusions from meat have been developed, giving much 

 better growth of these discriminating organisms. For isolation of the gonococcus 

 we recommend a Chocolate Agar prepared from Bacto-Proteose No. 3 Agar and 

 Bacto-Hemoglobin, enriched with Bacto-Supplement A or Bacto-Supplement B, 

 or Bacto-G. C. Medium Base similarly enriched. The procedure is given in de- 

 tail on pages 116 and 122. For cultivation of the gonococcus in pure culture we 

 recommend Bacto-Dextrose Starch Agar as discussed on page 124. 



To rehydrate the medium, suspend 53 grams of Bacto-Brain Veal Agar in 

 1000 ml. cold distilled water and heat to boiling to dissolve the medium com- 

 pletely. Distribute in tubes or flasks and autoclave for 15 minutes at 15 pounds 

 pressure (121°C.). The final reaction of the medium will be pH 7.6. 



