126 DIFCO MANUAL 



excellent medium for cultivating the meningococcus, gonococcus, Hemophilus 

 influenzae and H. pertussis. 



To rehydrate the medium, suspend 43 grams of Bacto-Dextrose Agar in 1000 

 ml. cold distilled water and heat to boiling to dissolve the medium completely. 

 Distribute in tubes or flasks and sterilize in the autoclave for 15 minutes at 15 

 pounds pressure (121°C.). Final reaction of the medium will be pH 7.3. 



Since best results are obtained with solid media having a moist surface, 

 Dextrose Agar should be used the same day as it is prepared. If not used at 

 once, melt the medium and allow to re-solidify just prior to inoculation. 



One pound of Bacto-Dextrose Agar will make 10.5 liters of medium. 



1 J. Lab. Clin. Med., 17:585:1932. 



BACTO 



BREWER ANAEROBIC AGAR (B279) 



DEHYDRATED 



Bacto- Yeast Extract 5 g 



Bacto-Tryptone 5 g 



Proteose Peptone No. 3, Difco 10 g 



Bacto-Dextrose 10 g 



Sodium Chloride 5 g 



Bacto-Agar 20 g 



Sodium Thioglycollate, Difco 2 g 



Sodium Formaldehyde Sulfoxylate . . 1 g 



Resazurin, Certified 0.002 g, 



Bacto-Brewer Anaerobic Agar is prepared for use with the Brewer anaerobic 

 cover as described by Brewer.^ This special petri dish cover was designed to 

 permit surface growth of anaerobes and micro-aerophiles on agar with a low 

 oxidation-reduction potential without the use of anaerobic jars or other special 

 apparatus. Brewer Anaerobic Agar is recommended to those laboratories wishing 

 to use this procedure for anaerobic culture. 



To rehydrate the medium, suspend 58 grams of Bacto-Brewer Anaerobic Agar 

 in 1000 ml. cold distilled water and heat to boiling to dissolve the medium 

 completely. Distribute in flasks and sterilize in the autoclave for 15 minutes at 

 15 pounds pressure (121°C.). Dispense the medium in petri dishes using 50-75 

 ml. of medium in 95 x 15-20 mm. petri dish bottoms. For best results use porous 

 tops on the dishes containing the medium during solidification in order to ob- 

 tain a dry surface. Inoculate the surface of the medium by smearing or streak- 

 ing. Cover the inoculated dish with a sterile Brewer anaerobic petri dish cover. 

 It is essential that the sealing ring inside the cover makes perfect contact with 

 the medium. This seal must not be broken before the end of the incubation 

 period. Poured plates may be made by placing the inoculum in the dish and mix- 

 ing with the medium before solidification. The final reaction of the medium 

 will be pH 7.2. 



One pound of Bacto-Brewer Anaerobic Agar will make 8.5 liters of medium. 



1 Science, 95:587:1942- 



