DEHYDRATED CULTURE MEDIA 127 



BACTO 



BREWER ANAEROBIC AGAR (B433) 

 without Dextrose or Eh Indicator 



DEHYDRATED 



Bacto-Brewer Anaerobic Agar without dextrose or Eh Indicator has the same 

 composition as Bacto-Brewer Anaerobic Agar except dextrose and resazurin have 

 been omited. This carbohydrate-free modification may be used for the Isolation 

 and growth of anaerobes and may be used as a base for Blood Agar, Upon the 

 addition of carbohydrates the medium may be employed for fermentation 

 studies. The addition of 1 per cent of the test carbohydrate is recommended for 

 these studies. 



To rehydrate the medium, suspend 48 grams Bacto-Brewer Anaerobic Agar 

 without Dextrose or Eh Indicator in 1000 ml. cold distilled water. Heat to 

 boiling to dissolve the medium completely. Distribute In tubes or flasks and 

 sterilize in the autoclave for 15 minutes at 15 pounds pressure (121°C.). Final 

 reaction of the medium will be pH 7.2. 



One pound of Bacto-Brewer Anaerobic Agar without dextrose or Eh Indicator 

 will make 9.4 liters of medium. 



BACTO 

 NUTRIENT AGAR 1.5% (B69) 



DEHYDRATED 



Bacto-Beef Extract 3 g. 



Bacto-Peptone 5 g. 



Sodium Chloride 8 g. 



Bacto-Agar 15 g. 



Bacto-Nutrlent Agar 1.5% is recommended as a slightly alkaline Nutrient 

 Agar, for use in the cultivation of bacteria not requiring a highly nutritious 

 medium. This medium contains 0.8 per cent sodium chloride, making it well 

 suited for the addition of blood, ascitic fluid or other enriching fluids. With 

 added food material, Bacto-Nutrient Agar 1.5% is satisfactory for the cultiva- 

 tion of pathogenic bacteria. 



Bacto-Blood Agar Base, Bacto-Tryptose Blood Agar Base or Bacto-Heart Infu- 

 sion Agar, as discussed on pages 88, 115 or 87, is recommended as being superior 

 to Bacto-Nutrient Agar 1.5% for use In making Blood Agar. For general culture 

 purposes, with or without enrichment, Bacto-Tryptose Agar, Bacto-Proteose No. 

 3 Agar, Bacto-Dextrose Proteose No. 3 Agar and Bacto-Dextrose Starch Agar, as 

 discussed on pages 111, 116, 147 and 124, respectively, are recommended as 

 being more satisfactory than Bacto-Nutrient Agar 1.5%. 



To rehydrate the medium, suspend 31 grams of Bacto-Nutrient Agar 1.5% in 

 1000 ml. cold distilled water and heat to boiling to dissolve the medium com- 

 pletely. Distribute In tubes or flasks and sterilize in the autoclave for 15 minutes 

 at 15 pounds pressure (121°C.). The final reaction of the medium will be pH 

 7.3. If enrichment Is desired, the freshly sterilized medium Is cooled to 45-50° C. 

 and sterile blood, ascitic fluid or other sterile serous enrichment is added. 



Since best results are obtained on a solid medium having a moist surface, 

 Nutrient Agar 1.5% should be used the same day as prepared, or, if not used 

 at once, the medium should be melted and allowed to re-solidify just prior to 

 inoculation. 



One pound of Bacto-Nutrient Agar 1.5% will make 14.6 liters of medium. 



