for 18-20 minutes at 15-17 pounds pressure (121-123°C.). Cool quickly to 

 25°C. The final reaction of the medium will be pH 7.1. 



One pound of Bacto-Fluid ThioglycoUate Medium will make 15.4 liters of 

 medium. 



^ National Institute of Health Circular : Culture 

 Media for the Sterility Test, and Revision, 

 Feb. 5, 1946. 



2 Compilation of Regulations for Tests and 

 Methods of Assay and Certification of Anti- 

 biotic Drugs, Federal Security Agency, Food 

 and Drug Administration. 



3 Pharmacopeia of the United States, XIV Re- 

 vision: 758: 1950. 



* National Formulary, 9th Edition: 768: 1950. 



6 J. Lab. Clin. Med., 32:1153:1947. 



<• J. Infectious Diseases, 29:390:1951. 



' J. Bact., 37:121:1939. 



sCentr. Bakt. I., Abt., 23:1038:1898. 



1940. 



;d. 43:672:1940. 



^ Biochem. J., 20:1125:1926. 



^0 J. Am. Med. Assoc, 115:; 



^ Proc. Soc. Exp. Biol. Mec 



12 Proc. Soc. Exp. Biol. Med. 52:287:1943. 



^ J. Bact., 48:255:1944. 



1* J. Bact., 62:349:1951. 



^ National Institute of Health. Fluid Thiogly- 

 coUate Medium for the Sterility Test, Dec. 

 30, 1941- 



M J. Bact., 48:256:1944- 



" Paper Read at New York Meeting of Am. 

 Pub. Health Assoc, 1944. 



18 J. Bact., 51:19:1946. 



BACTO 



BREWER THIOGLYGOLLATE MEDIUM 



DEHYDRATED 



(B236) 



BACTO 



LINDEN THIOGLYGOLLATE MEDIUM 



DEHYDRATED 



(B237) 



These media formerly specified by the National Institute of Health have been 

 used in the past for testing of sterility or other purposes. For those laboratories 

 desiring to continue to use these media Bacto-Brewer ThioglycoUate Medium 

 and Bacto-Linden ThioglycoUate Medium are carried in stock. 



