200 DIFCO MANUAL 



without Dextrose in 1000 ml. cold distilled water and heat to boiling to dissolve 

 the medium completely. Add selected carbohydrate, distribute in tubes and 

 sterilize in the autoclave for 15 minutes at 15 pounds pressure (121°C.). Final 

 reaction of the medium will be pH 7.2. 



One pound of Bacto-Thiogly collate Medium without Dextrose will make 19 

 liters of medium. 



1 J. Infectious Diseases, 29:390:1921. * J. Am. Med. Assoc, 115:598:1940. 



2Centr. Bakt., I Abt., 23:1038:1898. ^ Proc. Soc. Exp. Biol. Med., 43:672:1940. 



3 Biochem. J., 20:1125:1926. 



BACTO 



THIOGLYGOLLATE MEDIUM (B430) 

 without Indicator 



DEHYDRATED 



Bacto-Thioglycollate Medium without Indicator has the same composition as 

 Bacto-Thioglycollate Medium without Dextrose, described above except that 

 it contains 0.5 per cent Bacto-Dextrose and no methylene blue as an indicator 

 of Eh. 



To rehydrate the medium, suspend 29 grams Bacto-Thioglycollate Medium 

 without Indicator in 1000 ml. cold distilled water and heat to boiling to dis- 

 solve the medium completely. Distribute in tubes or flasks and sterilize in the 

 autoclave for 15 minutes at 15 pounds pressure (121°C.). Final reaction of the 

 medium will be pH 7.2. 



One pound of Bacto-Thioglycollate Medium without Indicator will make 

 15.6 liters of medium. 



BACTO 



THIOGLYGOLLATE MEDIUM (B432) 

 without Dextrose or Indicator 



DEHYDRATED 



Bacto-Thioglycollate Medium without Dextrose or Indicator has the same 

 composition as Bacto-Thioglycollate Medium without Dextrose as described 

 on page 199 except that it does not contain methylene blue as an indicator of Eh. 



To rehydrate the medium, suspend 24 grams Bacto-Thioglycollate Medium 

 without Dextrose or Indicator in 1000 ml. cold distilled water and heat to 

 boiling to dissolve the medium completely. Add selected carbohydrate, distribute 

 in tubes and sterilize in the autoclave for 15 minutes at 15 pounds pressure 

 (121°C.). Final reaction of the medium will be pH 7.2. 



One pound of Bacto-Thioglycollate Medium without Dextrose or Indicator 

 will make 19 liters of medium. 



BACTO 



SABOURAUD LIQUID MEDIUM (B382) 



DEHYDRATED 



Neopeptone, Difco 10 g. 



Bacto-Dextrose 20 g. 



Bacto-Sabouraud Liquid Medium is recommended as a liquid medium for the 

 cultivation of yeast, molds and aciduric and acidophilic bacteria. This medium 



