DEHYDRATED CULTURE MEDIA 201 



is particularly well suited for sterility test procedures for the detection of fungi. 

 It is prepared according to the formula specified in the U. S. Pharmacopeia^ and 

 National Formulary^ as used in sterility test procedures. The acid reaction of the 

 final medium, pH 5.7, is inhibitive to a large number of bacteria, but particu- 

 larly well suited for the cultivation of fungi and acidophilic microorganisms. 

 Neopeptone is used in the preparation of this medium since this peptone is 

 particularly well suited for the cultivation of fungi. 



To rehydrate the medium dissolve 30 grams Bacto-Sabouraud Liquid Medium 

 in 1000 ml. of distilled water. Distribute in tubes or flasks and sterilize in the 

 autoclave for 15 minutes at 15 pounds pressure (121°C.). Final reaction of the 

 medium will be pH 5.7. 



One pound of Bacto-Sabouraud Liquid Medium will make 15.1 liters of 

 medium. 



1 Pharmacopeia of the United States, XIV 2 National Formulary, gth Edition: 769: 1950. 

 Revision : 760 : 1 950. 



BACTO 



A G MEDIUM (B316) 



DEHYDRATED 



Bacto-Beef Extract 3 g. 



Bacto- Yeast Extract 3 g. 



Bacto-Malt Extract 3 g. 



Proteose Peptone No. 3, Difco 20 g. 



Bacto-Dextrose 5 g. 



Bacto-Agar 1 g. 



Ascorbic Acid 0.2 g. 



Bacto-A C Medium is recommended for use in controlling sterility of prod- 

 ucts in the process of manufacture and for testing sterility of solutions and other 

 materials not containing mercurial preservatives. For the sterility testing of bio- 

 logicals and solutions containing mercurials as a preservative, Bacto-Fluid Thio- 

 glycoUate Medium, page 195, should be employed. 



Bacto-A C Medium is an infusion-free medium possessing unique growth- 

 promoting properties for both aerobic and anaerobic microorganisms, making 

 possible an early and voluminous growth. It is recommended as a general 

 culture medium for the propagation of anaerobes, micro-aerophiles and aerobes. 

 Bacto-A C Medium does not exhibit the toxicity shown by media containing 

 sodium thioglycollate for some organisms as reported by Christensen,^ and Malin 

 and Finn.2 



Reed and Orr^ obtained excellent growth of all species of Clostridium on 

 Bacto-A C Medium. Schneiter, Dunn and Caminita* in their studies on the 

 bacterial content of air samples reported Proteose Extract Agar, the same com- 

 position as A C Medium with 0.85 per cent sodium chloride and 1.7 to 2.4 per 

 cent Bacto-Agar added to be satisfactory for the growth of staphylococci and 

 sporulates, and superior to all other media tested for the growth of streptococci. 

 Bailey et al.^ employed A C Medium in assaying for potency of streptomycin 

 products using Clostridium perfringens as a test organism and reported excellent, 

 rapid results. Schneiter and Kolb^ used Bacto-A C Medium for the growth of 

 Bacillus anthracis and related mesophilic aerobic bacilli in their studies of the 

 heat resistance of these organisms from hair and bristles. They reported that the 

 medium permitted the distinctive cottonball appearance of the anthrax colonies. 

 Kolb and Schneiter^ used Bacto-A G Medium to test the viability of B. anthracis 

 following exposure to methyl bromide to test the efficiency of this compound as 

 a germicidal and sporicidal agent. 



