DEHYDRATED CULTURE MEDIA 239 



One pound of Bacto-Sabouraud Dextrose Agar will make 6.9 liters of medium. 



lAnn. dermatol. syphilol., 1892-1893. "Pub. Health Reports, 66:1533:1951. 



2 Proc. Soc. Exp. Biol. Med., 38:700:1938. ^j Bact., 62:613:1951. 



8 Pub. Health Reports. 63:1416:1948. 'Derm. Wschr., 124:663:1951. 



* Arch. Dermatol Syphilol., 52:257:1945. 



BACTO 



SABOURAUD MALTOSE AGAR (BllO) 



DEHYDRATED 



Neopeptone, Difco 10 g. 



Maltose, Difco 40 g. 



Bacto-Agar 15 g, 



Bacto-Sabouraud Maltose Agar is a modification of the formula suggested by 

 Sabouraud.i Comparative tests have shown that Neopeptone, Difco is a most 

 satisfactory source of nitrogen for the development of fungi. This medium is an 

 excellent substrate for the propagation of molds and yeasts, particularly the 

 parasitic fungi concerned with skin and scalp lesions. 



Bacto-Sabouraud Maltose Agar, like Bacto-Sabouraud Dextrose Agar, contains 

 no selective agent, and depends entirely on the acid reaction, pH 5.6, for the 

 selective growth of fungi over bacteria. In the initial cultivation of fungi from 

 specimens many investigators prefer to use a selective medium such as Bacto- 

 Littman Oxgall Agar, as discussed on page 240 or Bacto-Brain Heart Infusion 

 Agar as described on page 90. 



Antibiotics may also be added to Sabouraud Maltose Agar to give selective 

 media, as discussed under Bacto-Sabouraud Dextrose Agar immediately above. 



The use of maltose, or the extractives of malt, in media designed for the 

 cultivation of molds and other fungi is quite universal. Maltose is well adapted 

 to the nutritional requirements of these organisms. 



Frank^ has used Bacto-Sabouraud Maltose Agar successfully in cultivating the 

 causative organisms of perleche. Davidson, Dawding and Buller^ reported that 

 Bacto-Sabouraud Maltose Agar was a most satisfactory medium in their studies 

 of the infections caused by Microsporon audouini, M. lanosum and Trichophyton 

 gypseum. Davidson and Dawding^ also used this medium in isolating T. gypseum 

 from a case of tinea barbae. Serowy and Jung^ used Bacto-Sabouraud Maltose 

 Agar in their study of the Microspora and called attention to the suitability of 

 this medium for the cultivation of Microspora and other pathogenic fungi, as well 

 as the ease with which this medium may be prepared and used. A. W. Bengtson^ 

 observed that Sabouraud Maltose Agar could be used to advantage in the isola- 

 tion and differentiation of Pseudomonas. On this medium the blue pyocyanin 

 pigment is enhanced making it easy to determine pigment production thereby 

 detecting Pseudomonas organisms in mixed infections. Sabouraud Dextrose Agar 

 on the other hand, tends to elicit the production of the pink fluorescene pigment 

 with suppression of the pyocyanin. Chapman^ modified Sabouraud Maltose Agar 

 in the preparation of a selective medium for the isolation and identification of 

 Monilia and other fungi. The medium was prepared by adding 0.1 ml. of Tergitol 

 7 and 0.0025 per cent Brom Cresol Purple to Sabouraud Maltose Agar at 

 pH 5.6. The medium was sterilized in the autoclave for 10 minutes and when 

 cooled to 45-55 °C., 0.3 ml. of Bacto-Chapman Tellurite Solution and 3 ml. of 

 2,3,5-triphenyltetrazolium chloride (TTC), was added. Surface of the plates 

 were inoculated followed by incubation at 37°C. for 48 hours. Chapman reported 

 that the Tergitol 7 inhibited all bacteria except members of the coliform group, 

 while the potassium tellurite inhibited these organisms. Candida albicans pro- 



