240 DIFCO MANUAL 



duced "off white" circular smooth entire convex to pulvinate colonies about 

 4 mm. in diameter. Other Candida produce colored colonies ranging from orange 

 to tan to lilac, often discoloring the medium. Sac char omyces grew in 48 hours 

 on the medium producing colonies somewhat resembling those of Candida. 



To rehydrate the medium, suspend 65 grams of Bacto-Sabouraud Maltose 

 Agar in 1000 ml. of cold distilled water and heat to boiling to dissolve the 

 medium completely. Distribute in tubes or flasks, and sterilize in the autoclave for 

 15 minutes at 15 pounds pressure (121°C.). The final reaction of the medium 

 will be pH 5.6. 



One pound of Bacto-Sabouraud Maltose Agar will make 6.9 liters of medium. 



1 Ann. dermatol. syphilol., 1892-1893. ^ Derm. Wschr., 124:665:1951. 



2 Arch. Dermatol. Syphilol., 26:451:1932. ^ Personal Communication, 1951. 



8 Can. J. Research, 6:1 : 1932. 'Trans. New York Acad. Sci. Series II, 



* Arch. Dermatol. Syphilol., 26:660:1932. 14:254:1952. 



BACTO 



SABOURAUD MALTOSE BROTH (B429) 



DEHYDRATED 



Neopeptone, Difco 10 g. 



Maltose, Difco 40 g. 



Bacto-Sabouraud Maltose Broth is a liquid medium recommended for the culti- 

 vation of fungi. It is acid in reaction and has the same formula as Bacto- 

 Sabouraud Maltose Agar except agar is omitted. Bacto-Sabouraud Maltose Broth 

 is also well suited for the detection of fungi in sterility test procedures. 



To rehydrate the medium, dissolve 50 grams Bacto-Sabouraud Maltose Broth 

 in 1000 ml. of distilled water. Distribute in tubes or flasks and sterilize in the 

 autoclave for 15 minutes at 15 pounds pressure (121°G.). Final reaction will 

 be pH 5.6. 



One pound of Bacto-Sabouraud Maltose Broth will make 9 liters of medium. 



BACTO 



SABOURAUD LIQUID MEDIUM 



DEHYDRATED 



Bacto-Sabouraud Liquid Medium is recommended as a liquid medium for the 

 cultivation of fungi. This medium supports early and luxuriant growth of fungi 

 from small inocula. A complete discussion of this medium is given on page 200. 

 A selective liquid medium may be prepared by the addition of penicillin and 

 streptomycin as described in the discussion on Bacto-Sabouraud Dextrose Agar 

 page 238. 



BACTO 



LITTMAN OXGALL AGAR (B294) 



DEHYDRATED 



Bacto-Peptone 10 g. 



Bacto-Dextrose 10 g. 



Bacto-Oxgall 15 g. 



Bacto-Agar 20 g. 



Bacto-Crystal Violet ; 0.01 g. 



