DEHYDRATED CULTURE MEDIA 243 



BACTO 



MYGOLOGIGAL AGAR (B405) 



DEHYDRATED 



Bacto-Soytone 10 g. 



Bacto-Dextrose 10 g. 



Bacto-Agar 15 g. 



Bacto-Mycologlcal Agar is a medium which is neutral in reaction, recom- 

 mended for the cultivation of fungi. Better growth of many fungi is obtained on 

 neutral or slightly alkaline media. This medium is also recommended for carry- 

 ing stock cultures of fungi and for chlamydospore production. A selective medium 

 for fungi may be prepared from Bacto-Mycological Agar by the addition of peni- 

 cillin and streptomycin. Generally 20 units of penicillin and 40 micrograms of 

 streptomycin per ml. of medium are added to the sterile melted medium at 

 45-50° C. under aseptic conditions. A method to obtain these concentrations of 

 the antibiotics is given in the description of Bacto-Sabouraud Dextrose Agar page 

 238. This medium is also well suited as a Dextrose Agar for the cultivation of a 

 large variety of; :;aprophytic and pathogenic microorganisms. 



To rehydrate ihe medium, suspend 35 grams of Bacto-Mycological Agar in 

 1000 ml. of cold distilled water. Heat to boiling to dissolve the medium com- 

 pletely. Distribute in tubes or flasks and sterilize in the autoclave for 15 minutes 

 at 15 pounds pressure. Final reaction of the medium will be pH 7.0. 



One pound of Bacto-Mycological Agar will make 13 liters of medium. 



BACTO 



MYGOLOGIGAL BROTH (B406) 



DEHYDRATED 



Bacto-Soytone 10 g. 



Bacto-Dextrose 40 g. 



Bacto-Mycological Broth is a liquid medium neutral in reaction recommended 

 for the cultivation of fungi. A selective liquid medium for fungi may be prepared 

 by the addition of penicillin (generally 20 units per ml. medium) and strepto- 

 mycin (40 micrograms per ml. medium) or other selective agents to the sterile 

 medium at 50° C. or less under aseptic conditions. A method to obtain these 

 concentrations of the antibiotics is given in the description of Bacto-Sabouraud 

 Dextrose Agar page 238. Bacto-Mycological Broth is also well suited as a sterility 

 test medium for the detection of fungi. 



To rehydrate the medium, dissolve 50 grams Bacto-Mycological Broth in 1000 

 ml. distilled water. Distribute in tubes or flasks and sterilize in the autoclave for 

 15 minutes at 15 pounds pressure (121°G.). Final reaction of the medium will 

 be pH 7.0. 



One pound of Bacto-Mycological Broth will make 9.0 liters of medium. 



BACTO BACTO 



POTATO DEXTROSE AGAR MALT AGAR 



DEHYDRATED DEHYDRATED 



Bacto-Potato Dextrose Agar and Bacto-Malt Agar are recommended for the 

 cultivation of yeasts and molds and for counts of these organisms. A complete 

 discussion of these media is given on page 64, 65. 



