246 DIFGO MANUAL 



Distribute in tubes or flasks and sterilize in the autoclave for 15 minutes at 15 

 pounds pressure (121°C.). Final reaction of the medium will be pH 7.3. 

 One pound of Bacto-Czapek Solution Agar will make 9.2 liters of medium. 



1 Thorn and Church, The Aspergilli, 39:1926. 2 fhom and Raper, Manual of the Aspergilli, 



39:1945. 



BACTO 



MILDEW TEST MEDIUM (B428) 



DEHYDRATED 



Sodium Nitrate 3 g. 



Dipotassium Phosphate 1 g. 



Magnesium Sulfate 0.25 g. 



Potassium Chloride 0.25 g. 



Bacto-Agar 10 g. 



Bacto-Mildew Test Medium is recommended for use in all procedures for test- 

 ing processed fabrics, threads and cordage for mildew proofing. This medium is 

 also recommended as a solid basal medium of known chemical composition with 

 sodium nitrate as the sole source of nitrogen to which various sources of carbon 

 may be added to complete the medium. 



To rehydrate the medium, suspend 14.5 grams of Bacto-Mildew Test Medium 

 in 1000 ml. of cold distilled water. Heat to boiling to dissolve the medium com- 

 pletely. Distribute in tubes or flasks and sterilize in the autoclave for 15 minutes 

 at 15 pounds pressure (121°C.). Final reaction of the medium will be pH 6.8. 



One pound of Bacto-Mildew Test Medium will make 31.3 liters of medium. 



BACTO 



CORN MEAL AGAR (B386) 



DEHYDRATED 



Corn Meal, Infusion from 50 g. 



Bacto-Agar 15 g. 



Bacto-Corn Meal Agar is recommended for the production of chlamydospores 

 by Candida albicans and for the cultivation of phytopathological and other fungi. 

 The medium is prepared from an infusion of ground yellow corn, and solidified 

 by the addition of 1.5 per cent Bacto-Agar. A similar medium, with the addition 

 of 0.2 per cent Bacto-Dextrose, Bacto-Corn Meal Agar with Dextrose, will pro- 

 duce a more luxuriant growth of some fungi. 



To rehydrate the medium, suspend 17 grams of Bacto-Corn Meal Agar in 

 1000 ml. cold distilled water and heat to boiling to dissolve the medium com- 

 pletely. Distribute in tubes or flasks and sterilize in the autoclave for 15 minutes 

 at 15 pounds pressure (121°C.). Final reaction of the medium will be pH 6.0. 



One pound of Bacto-Corn Meal Agar will make 26.7 liters of medium. 



BACTO 



CORN MEAL AGAR (B114) 

 with Dextrose 



DEHYDRATED 



Corn Meal, Infusion from 50 g. 



Bacto-Dextrose 2 g. 



Bacto-Agar 15 g. 



