DEHYDRATED CULTURE MEDIA 247 



Bacto-Corn Meal Agar with Dextrose is recommended for the cultivation of 

 phytopathological and other fungi. It is prepared from an infusion of ground 

 yellow com to which is added 0.2 per cent Bacto-Dextrose and 1.5 per cent 

 Bacto-Agar. A similar medium prepared without added dextrose, Bacto-Corn 

 Meal Agar, is recommended for chlamydospore production by Candida albicans. 



To rehydrate the medium, suspend 19 grams of Bacto-Corn Meal Agar with 

 Dextrose in 1000 ml. cold distilled water and heat to boiling to dissolve the 

 medium completely. Distribute in tubes or flasks and sterilize in the autoclave 

 for 15 minutes at 15 pounds pressure (121°C.). Final reaction of the medium 

 will be pH 6.0. 



One pound of Bacto-Corn Meal Agar with Dextrose will make 23.8 liters of 

 medium. 



BACTO 



BEAN POD AGAR (B116) 



DEHYDRATED 



Green String Beans, Infusion from. . 200 g. 

 Bacto-Agar 15 g. 



Bacto-Bcan Pod Agar is recommended for the cultivation of phytopathological 

 and other fungi. It is prepared from an infusion of fresh green beans, and solidi- 

 fied with 1.5 per cent Bacto-Agar. 



Edgerton^ reported the value of fresh green bean pod infusion in media for the 

 cultivation of phytopathological fungi. He described a method of drying green 

 bean pods and storing the dry bean meal so as to have it available for the prepara- 

 tion of media at all seasons of the year. Bacto-Bean Pod Agar prepared from 

 fresh green beans possesses all the growth properties of fresh bean pod infusion, 

 and eliminates the necessity of preparing and storing the desiccated bean meal. 



To rehydrate the medium, suspend 22.5 grams of Bacto-Bean Pod Agar in 

 1000 ml. cold distilled water and heat to boiling to dissolve the medium com- 

 pletely. Distribute in tubes or flasks and sterilize in the autoclave for 15 minutes 

 at 15 pounds pressure (121°C.). Final reaction of the medium will be pH 5.6. 



One pound of Bacto-Bean Pod Agar will make 20.1 liters of medium. 



1 Phytopathology, 8 : 445 : 1 9 1 8. 



BACTO 



LIMA BEAN AGAR (B117) 



DEHYDRATED 



Lima Beans, Infusion from 62.5 g. 



Bacto-Agar 15 g. 



Bacto-Lima Bean Agar is recommended for the cultivation of phytopathological 

 and other fungi. It is prepared from an infusion of dry lima beans, and is 

 solidified by the addition of 1.5 per cent of Bacto-Agar. This medium possesses 

 all the nutritive properties of an infusion of lima beans and has a final reaction 

 of pH 5.6, making it well suited for the growth of fungi. 



To rehydrate the medium, suspend 23 grams of Bacto-Lima Bean Agar in 

 1000 ml. cold distilled water and heat to boiling to dissolve the medium com- 

 pletely. Distribute in tubes or flasks and sterilize in the autoclave for 15 minutes 

 at 15 pounds pressure (121°C.). Final reaction of the medium will be pH 5.6. 



One pound of Bacto-Lima Bean Agar will make 19.7 liters of medium. 



