254 DIFCO MANUAL 



in 5 ml. amounts in sterile 16 mm. cotton stoppered tubes Filter sterilize 

 ufficient amounts of the desired vitainins for 100 ml. of ^^e Hnal medmm m 

 90 ml. of distilled water. The final medmm is prepared by addmg 4.5 ml. of the 

 sterile vitamin solution to each 0,5 ml. of the sterile basal medmm under aseptic 

 conditions and mixing thoroughly by shaking. The final reaction of the basal 

 medium will be pH 4.5. 



iTI S Dent Ae. Tech. Bull., No. 1029:1931. ^ l '^^^■' ^^a'^^^'-^^^^- 

 ^y-Tropicil Med. Hyg., 42:i7^:x939. ^ J- B^^t.. 46:501:1943. 



a J. Bact., 52:293:1946. 



