INGREDIENTS OF CULTURE MEDIA 263 



description of this method is given on page 111 under the discussion of Bacto- 

 Tryptose Agar. 



Schuhardt, Rode, Foster and Oglesby,^^ by special techniques, demonstrated 

 that a few of the numerous samples of Bacto-Tryptose which had been in his 

 laboratory exhibited some toxicity for certain Brucella abortus strains used in his 

 laboratory. The particular samples of Bacto-Tryptose possessing this character- 

 istic had absorbed moisture and had undergone chemical change. Schuhardt^^ 

 in a discussion of this observation stated that "the ease of neutralization of this 

 toxic factor by blood, serum, agar and other substances tends to make the practi- 

 cal significance of the toxicity relatively minor. We probably would not have 

 encountered it had we not been doing extensive tests on the in vitro effect of 

 sulfonamides on Brucella using decimal dilution inocula." The high productivity 

 of Bacto-Tryptose Agar, and Bacto-Tryptose used clinically for the isolation and 

 cultivation of Brucella attests to its value for the primary cultivation of Brucella 

 as well as other fastidious organisms. 



A typical analysis of Bacto-Tryptose is given on page 265. 



^ Huddleson: Brucellosis in Man and Animals, ^ Standard Methods for the Examination of 

 14:1939- Water and Sewage, gth Edition: 193: 1946. 



2 J. Bact., 43:33:1942. 10 Am, J. Hyg., 40:136:1944. 



'^Am. J. Clin. Path. 17:281:1947. " Am. J. Pub. Health, 36:324:1946. 



J. Am. Water Works Assoc, 31:689:1939. 12 Am. J. Hyg., 41:211:1945. 



''Am. J. Pub. Health, 31:127:1941. ^ J. Bact., 46:343:1943. 



« Am. J. Pub. Health, 31:351:1941. " Proc. Soc. Expl. Biol. Med., 64:114:1947. 



"Am. J. Pub. Health, 33:"99:i943- "J- Bact., 57:1:1949. 



8 Am. J. Pub. Health, 34:735:1944. i« Personal Communication, 1949. 



NEOPEPTONE, DIFGO ( B 11 9 ) 



Neopeptone is an enzymatic protein digest especially adapted for the prepara- 

 tion of media to be used in the propagation of organisms usually considered 

 difficult to cultivate in vitro. Neopeptone has been shown to be particularly well 

 suited to the growth requirements of many delicate and fastidious bacteria. 



Dubos^ obtained growth of pneumococci from small inocula using media pre- 

 pared with Neopeptone. According to Dawson and his associates^-s.* the use of 

 Neopeptone facilitates the development of mucoid colonies of the hemolytic 

 streptococci. Long and Bliss^ consider Neopeptone to have definite advantages 

 over other peptones in media for the cultivation of the minute beta hemolytic 

 streptococci. Neopeptone media were also utilized by Lancefield^ in her work 

 on the classification of the streptococci. 



In addition to these studies, Neopeptone has been used with satisfaction for 

 the propagation of many other pathogenic organisms. Parker'^ used Neopeptone 

 media for the pneumococcus, and Bailey^ employed it in her medium for differ- 

 entiation of the gonococcus and meningococcus from other Neisseria. Spray^ used 

 Neopeptone in his media for classification of the sporulating anaerobes, Hobby^^ 

 employed it in her study of the diphtheria bacilli, and Eldering and Kendrick^^ 

 reported good results with this peptone in cultivating Hemophilus pertussis. 

 Krumwiede^2 ^sed Neopeptone in media for cultivation of group A streptococci. 

 Engley and Snyder^^ cultivated Pasteurella tularensis in media prepared with 

 Neopeptone. Casman,^^ in a comparison of peptones used in the preparation of 

 fresh beef infusion agar for Blood Agar, reported Neopeptone to be best suited 

 for inclusion in the infusion base. 



This peptone has proved to be of decided value in media for the cultivation of 

 pathogenic fungi. Growth of these microorganisms is rapid and colonial formation 

 uniform and typical for the various types. Bacto-Sabouraud Dextrose Agar and 

 Bacto-Sabouraud Maltose Agar, prepared with Neopeptone, are discussed on 

 pages 238-240. 



