264 DIFCO MANUAL 



A typical quantitative analysis of Neopeptone, Difco, is given on page 265. 



ij. Exp. Med., 52:331:1930- ^ J- Bact., 34:645:1937- 



* Proc. Soc. Exp. Biol. Med., 3i:594:i934' ^ J- Bact., 32:135:1936. 



'Science, 80:296:1934. 10 J. Infectious Diseases, 57:186:1935. 



* J. Infectious Disesises, 62:138:1938. ^ Am. J. Pub. Health, 26:506:1936. 

 "J. Exp. Med., 60:619:1934. ^ J. Bact., 46:117:1943. 



* Proc. Soc. Exp. Biol. Med., 38:473:1938. ^^ j. Bact., 51:573:1946. 



'J. Exp. Med., 67:667:1938. "Am. J. Clin. Path., 17:281:1947. 



B AGTO-CASITONE ( B259 ) 



Bacto-Casitone is a pancreatic digest of casein, conforming to the specifications 

 given by the National Institute of Health^ and the U. S. Pharmacopeia. ^ It is 

 recommended for use in the preparation of media for sterility testing according 

 to the National Institute of Health,^ "The compilation of Tests and Methods of 

 Assay for Antibiotic Drugs,"^ Federal Security Agency, Food and Drug Adminis- 

 tration, National Formulary,* and the U. S. Pharmacopeia.^ It is also recom- 

 mended for the preparation of media where an enzymatic hydrolyzed casein is 

 desired, as for example the liquid medium for the cultivation of Mycobacterium 

 tuberculosis as described by Dubos and Middlebrook.^ 



Bacto-Casitone is readily soluble in distilled water giving a clear nearly color- 

 less solution, neutral in reaction. It has a high tryptophane content making it 

 satisfactory for the detection of indole production. 



1 National Institute of Health Circular: Culture Assay for Antibiotic Drugs, Federal Security 

 Media for the Sterility Test, 2nd Revision: Agency, Food and Drug Administration. 

 February 5, 1946. * National Formulary, 9th Edition: 768: 1950. 



2 Pharmacopeia of the United States, XIV ^ Pharmacopeia of the United States, XIV 

 Revision: 075: 1950. Revision:758: 1950. 



>The compilation of Tests and Methods of ^ Am. Rev. Tuber., 56:335:1947. 



BAGTO-PEPTONIZED MILK (B35) 



Bacto-Peptonized Milk, as the name implies, is a skim milk digest. Inasmuch 

 as this product contains carbohydrates as well as nitrogenous materials it is con- 

 sidered as a complete medium. Bacto-Peptonized Milk is discussed in detail on 

 page 75. 



BAGTO-SOYTONE (B436) 



Bacto-Soytone is an enzymatic hydrolysate of soybean meal prepared under 

 controlled conditions especially for use in microbiological procedures. It is 

 recommended for use in media for the cultivation of a large variety of organisms, 

 including fungi, and is also used in media for microbiological assay. This peptone 

 contains the naturally occurring carbohydrate of the soybean and is suitable in 

 all microbiological procedures wherein carbohydrates are not objectionable. 

 Bacto-Soytone is completely soluble in distilled water in concentrations generally 

 employed and forms clear solutions with a neutral reaction. 



BACTO-PROTONE (B125) 



Bacto-Protone was developed in response to a demand for a product high in 

 proteoses which could be used in preparing varying combinations of proteoses, 

 peptones and amino acids in studies of bacterial metabolism. About 85 per cent 

 of the nitrogen in Bacto-Protone is precipitated by saturation with ammonium 

 sulfate, thus indicating a high content of proteoses. Until pure primary or 



