\ 



\ 

 INGREDIENTS OF CULTURE MEDIA 273 



a perfect suspension of the blood in the medium without the formation of air 

 bubbles. Broth media may be enriched in the same manner except that the 

 temperature of the mixing may be disregarded. 



^ J. Lab. Clin. Med., 16:166:1930. 



BAGTO-BEEF BLOOD SERUM (B138) 



Bacto-Beef Blood Serum is a dehydrated powdered form of fresh beef blood 

 serum. In a 10 per cent solution it is equivalent to fresh serum. 



Bacto-Beef Blood Serum is a useful aid to bacteriologists who have occasional 

 use for serum media. The use of this product eliminates all the disagreeable and 

 time-consuming procedures attendant upon the preparation of fresh serum. Bacto- 

 Beef Blood Serum may be employed with excellent success in the preparation 

 of many serum media. When it is used in plain 10 per cent solution or as an 

 ingredient of Loeffler Medium it forms firm, smooth, white slants upon heat 

 coagulation. 



The Beef Blood Penetration Test of the Institute of Paper Chemistry in their 

 Institute Tentative Method No. 522, November, 1941, specifies the use of a 10 

 per cent solution of Bacto-Beef Blood Serum in distilled water adjusted to pH 

 6.6 with a 5 per cent solution of phosphoric acid for the performance of their 

 test. 



BACTO-BEEF SERUM (B260) 



Bacto-Beef Serum is filter sterilized fresh normal beef serum. It is recom- 

 mended as an enrichment for use in bacteriological culture media, and in other 

 procedures requiring beef serum. Bacto-Beef Serum is supplied in packages of 

 one-half dozen and one dozen ampuls of 10 ml. each. 



BAGTO-HORSE SERUM (B261) 



Bacto-Horse Serum is prepared from normal horse serum. It is filter sterilized 

 and is recommended for use as an enrichment in bacteriological culture media 

 and in other procedures requiring horse serum. Bacto-Horse Serum is supplied 

 in packages of one-half dozen and one dozen ampuls of 10 ml. each. 



BAGTO-HORSE SERUM SALINE 1-6 (B262) 



Bacto-Horse Serum Saline 1-6 is normal horse serum diluted 1 to 6 with 

 saline. It is filter sterilized and is especially recommended for use in the cultiva- 

 tion of Endamoeba histolytica according to the procedure discussed on page 97. 

 Bacto-Horse Serum Saline 1-6 is supplied in packages of one-half dozen and one 

 dozen ampuls of 10 ml. each. 



BAGTO-ASGITIG FLUID (B135) 



Bacto-Ascitic Fluid is prepared from serous fluids removed aseptically from 

 the peritoneal cavity of a number of individuals. It is filter sterilized and 

 packaged aseptically. The finished product is subjected to regular sterility tests 



