INGREDIENTS OF CULTURE MEDIA 275 



50 units per ml. medium). The enriched medium is then mixed to obtain an 

 even distribution of all components, and distributed into tubes or plates as 

 desired. When solidified in tubes or plates, it is ready for surface inoculation. 



Bacto-Dubos Oleic Serum Complex is supplied in packages of one-half dozen 

 tubes of 20 ml. each. 



1 Am. Rev. Tuberculosis, 56:334:1942. 



BACTO-DUBOS OLEIC ALBUMIN COMPLEX (B375) 



Bacto-Dubos Oleic Albumin Complex is a sterile liquid enrichment for use 

 with Bacto-Dubos Oleic Agar Base for the preparation of a medium for the 

 isolation and cultivation of Mycobacterium tuberculosis, as described by Dubos 

 and Middlebrook.^ The enrichment consists essentially of a 0.05 per cent 

 solution of alkalinized oleic acid in a 5 per cent solution of albumin fraction V 

 in normal saline (0.85 per cent sodium chloride solution). 



A discussion of the complete medium is given under Bacto-Dubos Oleic Agar 

 Base, page 107. The medium is prepared by adding 20 ml. of Bacto-Dubos 

 Oleic Albumin Complex and 5,000 to 10,000 units of penicillin (25 to 50 units 

 per ml. medium) to 180 ml. of sterile rehydrated Bacto-Dubos Oleic Agar Base 

 at 50-55 °C. under aseptic conditions. The desired concentration of penicillin 

 may be readily obtained by dissolving the contents of one vial of penicillin 

 containing 100,000 units in 10 ml. sterile distilled water. One-half (0.5) ml. 

 (5,000 units) is added to 200 ml. of the sterile medium at 50-55 °C. under 

 aseptic conditions to obtain a concentration of 25 units per ml. medium ( 1 ml. 

 to give 50 units per ml. medium). The enriched medium is then mixed to 

 obtain an even distribution of all components, and distributed into tubes or 

 plates as desired. When solidified in tubes or plates, it is ready for surface 

 inoculation. 



Bacto-Dubos Oleic Albumin Complex is supplied in packages of one-half 

 dozen tubes of 20 ml. each. 



1 Am. Rev. Tuberculosis, 56:334:1942. 



BACTO-PEIZER TB MEDIUM ENRICHMENT (B401) 



Bacto-Peizer TB Medium Enrichm.ent is prepared according to the formula 

 described by Peizer and Schecter,^ and is used with Bacto-Peizer TB Medium 

 Base for the preparation of a medium for the isolation and cultivation of Myco- 

 bacterium tuberculosis. The enrichment consists of fresh egg yolk, glycerol, dex- 

 trose and malachite green as a selective agent. A complete discussion of the 

 medium is given on page 110. Bacto-Peizer TB Medium Enrichment is packaged 

 in 29 ml. amounts, sufficient for the preparation of 129 ml. of complete medium. 

 It is supplied in packages of one-half dozen vials. 

 ^Am. J. Clin. Path., 20:682:1950. 



BACTO-PPLO SERUM FRACTION (B441) 



Bacto-PPLO Serum Fraction is a sterile solution recommended as an enrich- 

 ment in media for the cultivation of PPLO (pleuropneumonia-like organisms). 

 It is employed in 1 per cent concentration in Bacto-PPLO Agar and Bacto- 

 PPLO Enrichment Broth as discussed on pages 89 and 82. Bacto-PPLO Serum 



