276 DIFGO MANUAL 



Fraction has also been used for the propagation, stabilization and maintenance 

 of the Treponemes in vitro. 



Bacto-PPLO Serum Fraction is the partially purified serum fraction required 

 for growth by the pleuropneumonia-like organisms described by Smith and 

 Morton.^ It has the advantage over sera or ascitic fluid in that only 1 per cent 

 is required for enriching broth or solid media for culturing PPLO and does not 

 exhibit the inhibitory effect which normal sera has upon some PPLO strains. 



Bacto-PPLO Serum Fraction was shown by Rose and Morton^ to replace 

 effectively the serum or albumin fractions commonly employed in the cultivation 

 of avirulent Treponemes. 



1 J. Bact. J 61:395:1951. 2 Am. J. Syphilis Gonorrh. Venereal Diseases, 



36:1:1952. 



BAGTO-SUPPLEMENT B (B276) 



Bacto-Supplement B is a sterile yeast concentrate for use in supplementing 

 media for culturing fastidious microorganisms with exacting growth require- 

 ments. It is particularly recommended for use in Chocolate Agar, prepared from 

 Bacto-G C Medium Base or Bacto-Proteose No. 3 Agar and Bacto-Hemoglobin, 

 employed in the cultural diagnosis of gonococcal infections. It is also recom- 

 mended for use as an enrichment in both solid and liquid media for the isolation 

 and propagation of Hemophilus influenzae as described by Neter.^'^ This supple- 

 ment, with crystal violet added (Bacto-Supplement A), may be recommended 

 for the preparation of selective enriched media for these organisms. 



Bacto-Supplement B is processed to preserve both the thermolabile and thermo- 

 stable growth accessory factors of fresh yeast, including glutamine, coenzyme 

 (v factor), cocarboxylase and other growth factors for the most exacting strains 

 of Neisseria gonorrhoeae and H. influenzae. It also contains the hematin or x 

 factor required by H. influenzae. 



Bacto-Supplement B is used in one per cent concentration in either solid or 

 liquid media. It is added aseptically after the medium has been sterilized in the 

 autoclave and cooled below 50° C. 



The use of this enrichment in media is discussed in detail under Bacto-G C 

 Medium Base, page 122, Bacto-Proteose No. 3 Agar, page 116 and Bacto-Brain 

 Heart Infusion, page 77. 



Bacto-Supplement B is supplied in packages of six bottles of 5 ml. each. 



1 Science, 106:350:1947. 2 j_ Bact., 54:70:1947. 



BACTO-SUPPLEMENT A (B246) 



Bacto-Supplement A is a sterile yeast concentrate containing crystal violet 

 as a selective agent for use in supplementing media for culturing fastidious micro- 

 organisms with exacting growth requirements. It is particularly recommended 

 for use in Chocolate Agar prepared from Bacto-Proteose No. 3 Agar or Bacto- 

 G C Medium Base and Bacto-Hemoglobin employed in the cultural diagnosis 

 of gonococcal infections. It is also suggested for use as a selective enrichment 

 in solid media containing Bacto-Hemoglobin for the isolation and propagation 

 of Hernophilus influenzae. 



Bacto-Supplement A is processed to preserve both the thermolabile and thermo- 

 stable growth accessory factors, including glutamine, coenzyme (v factor), 

 cocarboxylase, and other growth factors for the most exacting strains of Neisseria 

 gonorrhoeae and H. influenzae. It also contains the hematin or x factor required 



