278 DIFGO MANUAL 



BAGTO-MUELLER TELLURITE SERUM (B266) 



Bacto-Mueller Tellurite Serum is a sterile selective enrichment used in the 

 preparation of the tellurite plating medium described by Mueller and Miller^ 

 for the isolation of Corynebacterium diphtheriae. It is prepared according to the 

 directions given by the authors and contains lactate, pantothenate, beef serum 

 and potassium tellurite, as a selective agent. To prepare the complete medium, 

 add 25 ml. of Bacto-Mueller Tellurite Serum to 1000 ml. of sterile Bacto- 

 Mueller Tellurite Base at 50 °G. under aseptic conditions. Mix thoroughly avoid- 

 ing formation of air bubbles and distribute 20 ml. into sterile 95 mm. plates. 

 The resultant medium is transparent. A complete discussion of this medium is 

 given on page 149 under Bacto-Mueller Tellurite Base. 



Bacto-Mueller Tellurite Serum is supplied in single bottles or in packages of 

 six bottles of 25 ml. each. 



1 J. Bact., 51:743:1946. 



ENZYMES 



In this section are included enzymes generally used for the preparation of 

 Bacteriological culture media. We are pleased to present several new enzyme 

 preparations for microbiological and clinical laboratory procedures, including 

 Bacto-Penase, a potent Penicillinase, Bacto-Chicken Pancreas, for the liberation 

 of folic acid for microbiological assay, Bacto-Trypsin 1%, for Hemagglutination, 

 Bacto-Lysozyme and Bacto-Lysozyme Substrate. 



PANGESTIN, DIFGO, 1:75 (B150) 



{Pancreatic Enzymes) 



Pangestin is an active preparation of pancreatic enzymes. It is strongly 

 amylolytic, proteolytic, and lipolytic. It is also free from starch and sugar. One 

 part of Pangestin, Dif co, 1 : 75 will digest 75 parts of cooked potato starch under 

 the conditions of the U.S. P. test for amylase in pancreatin and will also digest 

 75 parts of casein under conditions of the U.S. P. test for trypsin in pancreatin. 

 The optimum reaction for pancreatic amylase is pH 7.1, for pancreatic trypsin 

 it is pH 7.8, and for pancreatic lipase it is pH 8.0. 



Pangestin, Difco, 1:75 is available in 500, 100 or 25 gram quantities. 



PEPSIN, DIFGO, 1:10,000 (B151) 



Pepsin is employed in the preparation of culture media solely because of its 

 ability to break down protein materials. One part of Pepsin, Difco, 1 : 10,000 will 

 digest 10,000 parts of freshly coagulated egg albumen under the conditions of 

 the U.S.P. test for pepsin. Pepsin acts best at a reaction of pH 1 .8. 



Pepsin, Difco, 1:10,000 is available in 500, 100 or 25 gram quantities. 



PAPAIN, DIFGO (B253) 



Papain, Difco, is prepared from the fruit of the papaya (Carica papaya). 

 It is recommended as a source of proteolytic enzyme of vegetable origin for the 

 digestion of meat and other protein materials. Papain has also been used in 



