282 DIFGO MANUAL 



the methods described by Wheeler, Luhby and Scholl,'^'^ Wiener and Katz^i or 

 by Rosenfield and Vogel.^^ 



Bacto-Trypsin 1% for Hemagglutination is supplied in packages of ten vials 

 of 10 ml. each. 



*Ztschr. Immunitats Exper. Therap., 45:223: ^ j, Jmujunol., 65:39: 1950. 



1925- . Am. Assoc. Blood Banks, 1951. 



2 Ztschr. Immunitats Exper. Therap., 52:85: i" Personal Communication, 1951. 



1927- "^ J. Immunol., 66:51:1951. 



8 The Thomsen Hemagglutination Phenomenon, '^ The Laboratory Digest, 15:5:1951. 



Levin & Munksgaard, 1930. " New York Academy of Sciences, 13:213:1951. 



* Brit. J. Exp. Path., 27:228:1946. 1* Am. J. Clin. Path., 21:635:1951. 



6 Nature, 158:880:1946. "J. Immunol., 68:97:1952. 

 « Nature, 159:779: i947- 

 ' Symposium on Serology of Blood Groups. Am, 



Assoc. Immunol., 1949. 



BAGTO-HEM AGGLUTINATION BUFFER (B454D) 



Bacto-Hemagglutination Buffer, prepared according to the formula of Wheeler, 

 Luhby and Scholl,^ is a desiccation consisting of sodium chloride, disodium phos- 

 phate and potassium dihydrogen phosphate. It is recommended for the prepara- 

 tion of isotonic buffered saline for use in hemagglutination studies. It is especially 

 recommended for rehydrating Bacto-Trypsin 1 % and for suspending and washing 

 red cells preparatory for hemagglutination. 



Bacto-Hemagglutination Buffer is rehydrated by dissolving 8.6 grams in 1000 

 ml. distilled water. The resultant solution is isotonic for blood cells and has a pH 

 of 7.3. 



Bacto-Hemagglutination Buffer is conveniently packaged in vials containing 

 8.6 grams, sufficient for one liter, and supplied in boxes of ten vials. 



1 J. Immunol., 65:39:1950. 



INVERTASE FOR ANALYTICAL USE (B154) 



Invertase for Analytical Use is an enzymatic extract of yeast cells. It is pack- 

 aged in ampuls of 10 ml. each of a standardized extract, 5 ml. of which will 

 cause complete inversion of 50 ml. of a 10 per cent sucrose solution in one hour 

 at room temperature. Invertase for Analytical Use is most active at a reaction 

 of pH 4.4-4.6. The K value of Invertase Analytical is K (A.O.A.G.) =0.1. 



Invertase for Analytical Use is supplied in packages of one-half dozen and one 

 dozen ampuls of 10 ml. each. 



BACTO 



CHICKEN PANCREAS (B459) 



DEHYDRATED 



Bacto-Chicken Pancreas is standardized desiccated chicken pancreas used in 

 the enzymatic liberation of folic acid from its conjugated state. It is prepared 

 according to the method described by the Official Agricultural Chemists.^ Bacto- 

 Chicken Pancreas, is recommended for use in techniques requiring this enzyme, 

 among which the following may be cited : 



Official Methods of Analysis of the Association of Official Agricultural Chem- 

 ists,2 Methods of Vitamin Assay,^ Sreenivasan, Harper and Elvehjem,* Burk- 



