INGREDIENTS OF CULTURE MEDIA 285 



The sterility testing of penicillin products for yeasts and molds is accomplished 

 by adding the test samples to Bacto-Sabouraud Liquid Medium and incubating 

 for 5 days at room temperature. The sterility testing of products for yeasts and 

 molds which have high concentrations of penicillin may require the addition of 

 Bacto-Penase Concentrate to the medium. 



When employed in media such as Bacto-Brain Heart Infusion with PAB and 

 Agar for culturing blood or other body fluids containing penicillin, it is recom- 

 mended that Bacto-Penase Concentrate be used in a concentration to give a final 

 unitage of 100 units or more per ml. of medium. 



Bacto-Penase Concentrate is stable when stored at refrigerator temperatures. 

 At normal room temperature slow deterioration of Bacto-Penase Concentrate 

 does occur, and for that reason this product, or media prepared with it, should 

 be stored at 2-6°C. 



Bacto-Penase Concentrate is regularly supplied in packages of one-half dozen 

 tubes of 20 ml. each or packages of one-half dozen bottles of 100 ml. each. 



1 Compilation of Regulations for Tests and biotic Drugs, Federal Security Agency, Food 



Methods of Assay and Certification of Anti- and Drug Administration. 



B AGTO-LYSOZ YME ( B465 ) 



BAGTO-LYSOZYME SUBSTRATE (B461) 



B AGTO-LYSOZ YME BUFFER (B464) 



Bacto-Lysozyme is a crystalline enzyme prepared from fresh egg white, con- 

 taining sufficient Bacto-Lysozyme Buffer to give a solution of pH 6.2 when re- 

 hydrated with distilled water. Bacto-Lysozyme Substrate is a dried standardized 

 irradiated culture of Micrococcus lysodeikticus recommended for use in the 

 turbidimetric assay of Lysozyme activity of body fluids, extracts from animal 

 tissues and other materials suspected of containing this enzyme. It is supplied 

 with sufficient Bacto-Lysozyme Buffer to give a suspension of pH 6.2 when re- 

 hydrated with distilled water. Bacto-Lysozyme Buffer, rehydrated in distilled 

 water is a phosphate solution at pH 6.2 used in the test. The test employs the 

 principles of Fleming,^ Goldsworthy and Florey,^ Boasson^ and Hartsell and 

 Smolelis.* A detailed discussion of the test is available upon request. 



1 Proc. Roy. Soc. (London), B, 93:306:1922. ^ J. Immunol., 34:281:1938. 



sBrit. J. Exp. Path., 11:192:1930. * J. Bact., 58:731 :»949' 



