TISSUE CULTURE REAGENTS 305 



TC Horse Serum is reconstituted for use by adding, aseptically, 4.5 ml. TC 

 Reconstituting Fluid or TC Triple Distilled Water to each bottle. The Reconsti- 

 tuting Fluid may be added conveniently with a sterile syringe and needle. Solu- 

 tion oi the material is accelerated by a gentle end-over-end rotation of the bottle. 

 Do not shake. When solution is complete, the serum is ready for use. Unused 

 portioas of the reconstituted TC Horse Serum should be kept in tightly stoppered 

 contaiiers in the refrigerator at 2-6°C. Storage of the reconstituted TC Horse 

 Serum for periods longer than one week, even though refrigerated, is not recom- 

 mended. 



• The reconstituted TC Horse Serum is alkaline in reaction due to loss of carbon 

 dioxide in the desiccation process and if desired may be adjusted to pH 7.2-7.4 

 in an atmosphere of carbon dioxide as described for the adjustment of Chicken 

 Plasma page 301. 



TC ASCITIC FLUID (B472) 



TC Ascitic Fluid is sterile, pooled, whole human ascitic fluid certified for use 

 in tissue culture procedures. It is obtained from cirrhotic patients, free from bile 

 salts, and is packaged in 10 ml. ampuls. TC Ascitic Fluid has a reaction of 

 pH 6.9-/.0 and requires adjustment to 7.2-7.4 before being used as a nutrient 

 for tissue cells. Adjustment is made by adding sterile sodium bicarbonate solu- 

 tion or by diluting the Ascitic Fluid with TC Balanced Salt Solution and agitating 

 the mixture gently to drive off some of the carbon dioxide. TC Phenol Red Solu- 

 tion 1% may be added to the Ascitic Fluid in a concentration of 0.002 per cent 

 as an indicator of reaction. (Add, aseptically, 0.02 ml. TC Phenol Red Solution 

 1% to 10 ml. Ascitic Fluid.) 



TC BEEF SERUM ULTRAFILTRATE (B473) 



TC Beef Serum Ultrafiltrate is a protein-free solution containing 0.001 per 

 cent Phenol Red indicator. It is prepared by the ultrafiltration of beef serum 

 through a colloidion membrane filter to remove all protein and preserve filterable 

 growth accessory factors. It is certified for use in tissue culture and is recom- 

 mended for all procedures requiring serum ultrafiltrate. TC Beef Serum ultra- 

 filtrate is especially suited as a basal substrate for culturing tissue cells, maintain- 

 ing tissue banks, propagating viruses and for maintaining the viability and 

 motility of Treponema pallida for the Treponema Immobilization test. It is 

 packaged in 50 ml. quantities in glass sealed ampuls. 



TC HORSE SERUM ULTRAFILTRATE (B474) 



TC Horse Serum Ultrafiltrate is a protein-free solution containing 0.001 per 

 cent Phenol Red indicator. It is prepared by the ultrafiltration of horse serum 

 through a colloidion membrane filter to remove all protein and preserve filterable 

 growth accessory factors. It is certified for use in tissue culture and is recom- 

 mended for all procedures requiring serum ultrafiltrate. TC Horse Serum ultra- 

 filtrate is especially suited as a basal substrate for culturing tissue cells, maintain- 

 ing tissue banks, propagating viruses and for maintaining the viability and 

 motility of Treponema pallida for the Treponema Immobilization test. It is 

 packaged in 50 ml. quantities in glass sealed ampuls. 



