306 DIFGO MANUAL 



TC BALANCED SALT SOLUTION (B351) 



TC Balanced Salt Solution is a sterile balanced salt solution certified br use 

 in tissue culture procedures. It is prepared according to the formula of larle,^ 

 and has the following composition: 



Sodium Chloride 6.8 g. 



Potassium Chloride 0.4 g. 



Calcium Chloride 0.2 g. 



Magnesium Sulfate 0.2 g. 



Sodium Phosphate Monobasic ... 0.125 g. 



Bacto-Dextrose 1. g. 



Sodium Bicarbonate 2.2 g. 



Triple Distilled Water 1,000 ml. 



TG Balanced Salt Solution is stabilized by adjustment of the reactior to pH 

 7.0 with carbon dioxide during preparation. It can readily be adjustec to pH 

 7.2-7.4 by permitting carbon dioxide to escape after removal from the bottle. 

 TC Phenol Red Solution 1% may be added to the TG Balanced Salt Solution 

 in 0.002 per cent concentration as an indicator of reaction. (Add, aseptically, 

 0.2 ml. TG Phenol Red Solution 1% to 100 ml. Balanced Salt Solution). TG 

 Balanced Salt Solution is packaged in 100 ml. bottles. 



1 Nat. Cancer Inst., 4:167:1943. 



TG RECONSTITUTING FLUID (B352) 



TG Reconstituting Fluid is sterile, triple distilled water saturated with carbon 

 dioxide and is certified for use in tissue culture procedures. It is packaged in 

 100 ml. quantities. It is used for reconstituting desiccated tissue culture reagents 

 where a lower pH is desired than obtainable through use of TG Triple Distilled 

 Water. 



TG TRIPLE DISTILLED WATER (B353) 



TC Triple Distilled Water is sterile and certified for use in tissue culture 

 procedures. It is the distillate from triple distillation of water in an all-Pyrex 

 still and is packaged in 100 ml. bottles. 



TG Triple Distilled Water is recommended as a reconstituting fluid for tissue 

 culture reagents and for the preparation of Balanced Salt Solution and other 

 media. 



TG PHENOL RED SOLUTION 1% (B358) 



TG Phenol Red Solution 1% is sterile and certified for use as an indicator of 

 pH in tissue culture procedures. It is employed in tissue culture media in con- 

 centrations of 0.001-0.002 per cent. For judging the pH of very small volumes 

 of media, (1-2 ml.), the concentration of phenol red should be 3-5 times that 

 given above, as suggested under the discussion of TG Chicken Plasma as given 

 on page 301. TG Phenol Red Solution is packaged in 1 ml. ampuls and supplied 

 in boxes of 6 ampuls. 



