308 DIFCO MANUAL 



the conventional size of the explants has approximated 1x1x1 mm., Earle has 

 used explants ranging from 0.5x1x2 mm. to 0.5x3x2 mm. In other cases he has 

 substituted cell suspensions for the explants. 



To a 35 mm. flask (Carrel type) add 0.3 ml. of TG Chicken Plasma, either 

 whole or diluted to 50 or even 25 per cent with TC Balanced Salt Solution, 

 and spread over bottom of flask. This is then increased in volume to 1 ml. by 

 introducing 0.6 ml. of TC Balanced Salt Solution and 0.1 ml. of TC Embryo 

 Extract EEgQ. Add explants and orient them. Stopper flasks and set aside to clot. 

 Incubate for 1-2 hours to give firm clot, then add 0.5 ml. of nutritional mixture 

 as described under Roller Tube Culture. 



Detailed methods of tissue culture may be found in texts such as those listed 

 below : 



Parker, Methods of Tissue Culture 

 Paul B. Hoeber, Inc., 1938, 1950. 



White, Handbook of Plant Tissue Culture 

 Ronald Press Company, 1943. 



Cameron Tissue Culture Technique 

 Academic Press, Inc., 1950. 



Pomerat, Methods in Medical Research Section IV, 1951 

 Year Book Publishers, 1951. 



