310 DIFCO MANUAL 



alcohol. The alcoholic extract of the tissue is fortified with 0.4 per cent Bacto- 

 Cholesterol and the ether-soluble, acetone-insoluble lipoids of the Bacto-Beef 

 Heart. The antigenic sensitivity of this antigen is similar to that of an antigen 

 prepared with 0.03 per cent cardiolipin, 0.05 per cent lecithin and 0.6 per cent 

 cholesterol when used in the Kolmer simplified and quantitative complement- 

 fixation tests for syphilis;^ the specificity of the antigens with sera and spinal 

 fluids of presumably normal nonsyphilitic individuals was reported to be equal. 

 The sensitivity of Bacto-Kolmer Improved Antigen (Reenforced) is slightly in- 

 creased over that of Bacto-Kolmer Improved Antigen. 



lAm. J. Clin. Path. 18:731:1948. 

 2 Texas State J. Mea., 44:312:1948, 



BACTO-EAGLE WASSERMANN (B214) 

 ANTIGEN 



Bacto-Eagle Wassermann Antigen was developed by Eagle^-- for use in his 

 modification of the Wassermann reaction. It is an alcoholic extract of Bacto-Beef 

 Heart reenforced with Bacto-Cholesterol. This antigen, when tightly stoppered, 

 retains its reactivity indefinitely at room temperature. 



1 J. Lab. Clin. Med., 22:300:1936. 

 3 Eagle: Laboratory Diagnosis of Syphilis, 

 144:1938. 



BACTO-B.J.L. WASSERMANN ANTIGEN (B234) 



Bacto-B.J.L. Wassermann Antigen is prepared according to the procedure 

 described by Boemer, Jones and Lukens^. The antigen is an alcoholic ex- 

 tract of Bacto-Beef Heart to which is added Bacto-Cholesterol. In the authors' 

 technique, the time and cost of performing the test is greatly reduced without 

 loss of specificity.2'3 It is suitable for quantitative as well as qualitative tests. The 

 antigen is stable when stored in tightly stoppered bottles at room temperature. 



1 Am. J. Clin. Path., 9:i3:i939- 



2 Arch. Derm. Syph., 41:32:1940. 



3 Am. J. Clin. Path., 10:282:1940. 



BACTO-ANTISHEEP HEMOLYSIN (B217) 



(Amboceptor) 



The epochal discovery of Bordet concerning the lysis of erythrocytes by 

 immunized serum is the foundation upon which the indicator system of the 

 complement-fixation test is built. 



Bacto-Antisheep Hemolysin (Amboceptor) is prepared by the method of 

 Kilduffe.^ It is a highly potent antisheep rabbit serum preserved with glycerol. 

 It is exceptionally stable and may be stored in the refrigerator for periods of 

 1 2 months or more without loss of activity if kept properly stoppered. 



^ J. Lab. Clin. Med., 10:582:1924-25. 



