312 DIFCO MANUAL 



BAGTO-KAHN SENSITIZED ANTIGEN (B219) 



Bacto-Kahn Sensitized Antigen is prepared in strict accordance with the pro- 

 cedure outlined by Kahn.^' 2,3,4 j^ |g ^j^ especially sensitive and stable antigen. 

 Each lot is carefully standardized and is rechecked under the personal super- 

 vision of Dr. Kahn. This antigen is used in the Kahn presumptive procedures 

 with serum and spinal fluid. To prevent fluctuations in the titer, this antigen 

 should be kept tightly stoppered, in the dark, at about 21 °C. (not below room 

 temperature). 



iRahn: The Kahn Test, A Practical Guide, 3 Am. J. Clin. Path., 17:130:1947. 



143:1928. * Am. J. Clin. Path., 17:770:1947. 



2 Kahn : Technique of the Standard Kahn 



Test, Univ. Mich. Press, 39:i944- 



BAGTO-EAGLE FLOGGULATION (B220) 

 ANTIGEN 



The flocculation technique developed and modified by Eagle^'2,3,4 {§ ^ Ye\2i' 

 tively simple and highly accurate diagnostic test for syphilis. Eagle's research on 

 the sterols led him to use a combination of corn germ sterol and cholesterol in 

 the antigen for his flocculation test. With these sensitizing agents he secured large 

 crystalline aggregates in positive sera, which made the reaction easier to read. 



Bacto-Eagle Flocculation Antigen is a sensitive and satisfactory reagent for use 

 in the Eagle flocculation technique. It is diluted for use according to the titer 

 shown on the label. The antigen is stable when stored at room temperature. As 

 described by Eagle^'* this same antigen is used in both the "macro" and "micro" 

 modification of his flocculation technique. 



1 J. Lab. Clin. Med., 17:787:1932. * Eagle: Laboratory Diagnosis of Syphilis, 



2 J. Lab. Clin. Med., 22:300:1936. 215:1937. 

 ^ Am. J. Syphilis, 22:22:1938. 



( 



BACTO-HINTON INDICATOR (B221) 



The Hinton Glycerol Cholesterol Reaction for the detection of syphilis is 

 recommended for its accuracy and simplicity. For the actual reading of the test 

 only one tube need be considered. Like all other serological diagnostic tests, how- 

 ever, the success of this test depends upon the sensitivity of the reagents used as 

 well as rigid adherence to the details of the technique. 



Bacto-Hinton Indicator is the cholesterolized heart extract used in Hinton's^ 

 Glycerol Cholesterol Reaction for syphilis, and also in "A Modification of the 

 Hinton Test applied to Spinal Fluid" by Davies.^ This extract has been prepared 

 according to the directions given by Hinton and is also recommended for use in 

 the "micro" and "capillary" serological tests as described by Davies.^ 



This indicator is stable when it is stored, tightly stoppered, at room tempera- 

 ture. In the event that the cholesterol precipitates, the indicator is warmed to 

 redissolve the cholesterol before diluting it with salt solution for use in the per- 

 formance of the test. 



Each lot of Bacto-Hinton Indicator is tested in Dr. Hinton's laboratory and is 

 not released for distribution until his approval is received. 



ij. Lab, Clin. Med., 18:198:1932-33. 



a Am. J. Clin. Path. 7:240:1937. 



•J. Lab. Clin. Med., 22:959:1936-37. 



