SEROLOGICAL REAGENTS 313 



BACTO-BJ.L. FLOGGULATION (B235) 

 ANTIGEN 



Bacto-B.J.L. Flocculation Antigen is prepared according to the directions given 

 by Boerner, Jones and Lukens.^ It is an alcoholic extract of Bacto-Beef Heart. 

 It is stored in the dark in a tightly stoppered bottle at room temperature. Bacto- 

 B.J.L. Flocculation Antigen is satisfactory for the performance of the micro- 

 scopic and macroscopic flocculation tests described by these authors. Their tech- 

 nique has the advantage of simplicity, reproducibility and a desirable degree of 

 sensitivity and specificity. 



1 Am. J. Clin. Path., Tech. Sup., 4:141:1940. 



GARDIOLIPIN ANTIGENS 



BAGTO-V D R L ANTIGEN (B388) 



Bacto-V D R L Antigen is a cardiolipin-lecithin antigen for the V D R L Slide 

 and Tube Test Procedures used in the serological diagnosis of syphilis. 



Bacto-V D R L Antigen is prepared according to the directions given by 

 Harris, Rosenberg, and Riedel,^ and consists of cardiolipin, cholesterol and 

 lecithin dissolved in absolute ethyl alcohol. Cardiolipin and lecithin are prepared 

 according to directions given by Pangborn.2'3.4.5 



Buffered saline used in dilution of the Bacto-V D R L Antigen is prepared 

 according to the directions given by Harris, Rosenberg, and Riedel.^ 



Bacto-V D R L Antigen is used in the techniques as given in detail in the 

 Manual of Serologic Tests for Syphilis, Federal Security Agency, Public Health 

 Service, Division of Venereal Disease.^ 



Bacto-V D R L Antigen is supplied in 5 ml. units. Each unit consists of 5 ml. 

 antigen and 50 ml. Buffered Saline for diluting the antigen in preparing the 

 emulsion for the VDRL Slide and Tube Test Procedures for the serological diag- 

 nosis of syphilis. 



1 J. Venereal Disease Inform., 27:169:1946. ^ j, giol. Chem., 161:71:1945. 



2 Proc. Soc. Exp. Biol. Med., 48:484:1941. « J. Venereal Disease Inform., Supplement 



3 J. Biol. Chem., 143:484:1942. No. 22, 1949. 

 *J. Biol. Chem., 153:343: i944- 



BAGTO-KAHN GARDIOLIPIN ANTIGEN (B404) 



Bacto-Kahn Cardiolipin Antigen is prepared according to the directions given 

 by Kahn and McDermott^ and Kahn^ and consists of cardiolipin, lecithin and 

 cholesterol in absolute ethyl alcohol. The cardiolipin and lecithin are prepared 

 according to the directions given by Pangborn.^'*'^-^ The amount of lecithin 

 present may vary slightly to give a suitable antigen; however, generally the anti- 

 gen consists essentially of 0.1 per cent cardiolipin, LO per cent lecithin, and 

 0.025 per cent cholesterol. 



Bacto-Kahn Cardiolipin Antigen is recommended for use in conjunction with 

 standard Kahn antigen in the performance of the Kahn Standard Test with 

 serum and spinal fluids as well as quantitative procedures in accordance with the 

 directions given by Kahn.^ This antigen may also be used in a microflocculation 

 procedure as described by Kahn and McDermott.^ Each batch of Bacto-Kahn 

 Cardiolipin Antigen is tested and approved in Dr. Kahn's laboratory to assure 

 an antigen of the correct specificity and sensitivity. 



