314 DIFGO MANUAL 



A complete discussion of the Kahn Cardiolipin Antigen and its use in the 

 serological diagnosis of syphilis is given in "Serology with Lipid Antigen with 

 Special Reference to Kahn and Universal Reactions" by Kahn.^ 



Bacto-Kahn Cardiolipin Antigen is supplied in 5 ml. bottles. 



1 Am. J. Clin. Path., 18:364:1948. *J, Biol. Chem., 143:484:1942. 



2 Kahn, Serology with Lipid Antigen, p. 159, ^ j giol. Chem., i53:343:i944- 

 164, 259, 263, and 283, 1950. ^J Biol. Chem., 161:71:1945. 



sProc. See. Biol. Med., 48:484:1941. 



BACTO-KOLMER CARDIOLIPIN (B438) 

 ANTIGEN 



Bacto-Kolmer Cardiolipin Antigen is used in the Kolmer Complement Fixa- 

 tion Test for Syphilis as described by Kolmer and Lynch. ^ It consists of a solu- 

 tion of purified cardiolipin, lecithin and cholesterol (0.03 per cent cardiolipin, 

 0.05 per cent lecithin and 0.6 per cent cholesterol) in absolute ethyl alcohol in 

 the optimum combination to give maximum sensitivity consistent with specificity 

 in tests with sera and spinal fluids. Cardiolipin and lecithin are prepared accord- 

 ing to directions given by Pangborn.-'^*-^ 



Bacto-Kolmer Cardiolipin Antigen is tested and approved by Dr. Kolmer's 

 laboratory before release, assuring an antigen of the proper sensitivity and spec- 

 ificity. The dose to employ as given on the label was determined by a method 

 of antigenic titration described by Kolmer arid Boerner.^ 



This method determines the "optimum dose" instead of "antigenic units," and 

 the dose as given on the label is the optimum amount to use. It corresponds to 

 10 to 20 antigenic units by the older method of titration. The titration should be 

 checked before use. If employed for any other method, the titrations should be 

 conducted, and the dose determined, in accordance with the method employed. 

 The dilution should be prepared in quantities sufficient for one day's use, and a 

 new dilution made daily. 



Bacto-Kolmer Cardiolipin Antigen is supplied in 5 ml. bottles. 



1 J. Venereal Disease Inform., 29:166:1948. ^ J. Biol. Chem., 161:71:1945. 



2 Proc. Soc. Exp. Biol. Med., 48:484:1941. ^ j^olmer and Boerner, Approved Laborator 



3 J. Biol. Chem., 143:484:1942. Technic, 4th Edition: 679: 1945. 

 *J. Biol. Chem., I53:343;i944- 



BACTO-HINTON CARDIOLIPIN (B440) 

 INDICATOR 



Bacto-Hinton Cardiolipin Indicator is prepared according to the directions 

 given by Hinton, Stuart and Grant^ consisting of purified cardiolipin, lecithin 

 and cholesterol in absolute ethyl alcohol. The cardiolipin and lecithin are pre- 

 pared according to the directions given by Pangborn.2'3.4.5 



Bacto-Hinton Cardiolipin Indicator is recommended for the regular Hinton 

 qualitative and quantitative tests. Each batch of Bacto-Hinton Cardiolipin Anti- 

 gen is tested and approved by Dr. Hinton's laboratory before being released for 

 distribution. 



Bacto-Hinton Cardiolipin Indicator is supplied in 5 ml. bottles. 



1 Am. J. Syphilis Gonorrh. Venereal Dis- » j. gjol. Chem., 143:484:1942. 



eases, 33:587:1949. * J. Biol. Chem., 153 = 343:1944 



" Proc. Soc. Biol. Med., 48:484:1941. ^ j, gjol. Chem., 161:71:1945. 



