322 



DIFGO MANUAL 



solution for running prothrombin times of plasma collected and handled 

 in silicone coated glassware. 



12. Time of drawing blood. 



Blood drawn for prothrombin determinations should be taken previous to 

 meal time and not directly after meals. High lipid content of plasma 

 following ingestion of high fat food materials may prolong the clotting 

 time. 



13. Effect of Salicylates and other drugs. 



Aspirin and other salicylates, as well as caffeine as shown by Link,^* when 

 ingested may influence the clotting mechanism by prolonging the pro- 

 thrombin time. 



1 J. Biol. Chem., logrlxxiii: 1935. 



2 J. Am. Med. Assoc, 110:1658:1938. 



3 J. Hem., 4:1281:1949. 



* J. Exp. Med., 66:801:1937. 



s J. Am. Med. Assoc, 113:380:1939. 



* Am. J. Clin. Path., 10:147:1940. 

 'J. Am. Med. Assoc, 114:749:1940. 

 8 J. Biol. Chem., 138:1:1941- 



» Proc Soc Exp. Biol. Med., 50:85:1942. 

 ^0 Exp. Med. Surgery, 2:103:1944. 



11 Proc Soc. Exp. Biol. Med., 52:12:1943. 



12 Proc Soc. Exp. Biol. Med., 53:218:1943. 



13 Arch. Surgery, 50: 137:1 945- 

 i*Am. J. Clin. Path., 17:405:1947. 

 15 Am. J. Clin. Path., 19:471:1949. 



i« J. Am. Med. Assoc, 114:1342:1940. 

 "Am. J. Clin. Path., 19:1016:1949. 



942- 

 942. 



1944- 



18 Quick, The Physiology and Pathology of 



Hemostasis, 1951. 

 ""^ J. Am. Med. Assoc, 120:1009:1942. 

 Am. Med. Assoc, 120:1015 

 Am. Med. Assoc, 120:1025 

 ==> Minnesota Med., 27:455:1944. 

 23 New England J. Med., 230:13 

 ^ Surgery, 17:207:1945. 

 25 J. Am. Med. Assoc, 130:398:1946. 

 20 Minnesota Med., 29:250:1946. 

 f. Am. Med. Assoc, 134:323:1947. 

 icumarol Leaflet, Eli Lilly & Go. 

 28 Dicumarol Leaflet, Abbott Laboratories. 



30 Dicumarol Leaflet, E. R. Squibb & Sons. 



31 Proc Mayo Clinic, 26:257:1951. 

 62 Lancet, Vol. I, (XXIV) : 1133:1950. 



33 Personal Communication, 1950. 



34 Chicago Med. Bull., 51:53:1948. 



20 7 



28 Die 



BAGTO-SODIUM CHLORIDE 0.85 per cent (B379) 



Bacto-Sodium Chloride 0.85 per cent is recommended for use in all prothrom- 

 bin techniques requiring 0.85 per cent sodium chloride solutions. It is prepared 

 with reagent grade sodium chloride and triple distilled water. 



Bacto-Sodium Chloride 0.85 per cent is supplied in bottles of 100 ml. in 

 packages of 6. 



BAGTO-SODIUM OXALATE 0.1 MOLAR (B377) 



Bacto-Sodium Oxalate 0.1 Molar is recommended for use in all prothrombin 

 techniques requiring 0.1 Molar sodium oxalate solutions. It is prepared with 

 anhydrous, reagent grade sodium oxalate in triple distilled water. 



Bacto-Sodium Oxalate 0.1 Molar is supplied in 25 ml. bottles in packages of 6. 



BAGTO-GALGIUM GHLORIDE (B378) 

 0.02 MOLAR 



Bacto-Calcium Chloride 0.02 Molar is recommended for use in all prothrom- 

 bin techniques requiring 0.02 Molar solutions of calcium chloride. It is prepared 

 with anhydrous, reagent grade calcium chloride and triple distilled water. 



Bacto-Calcium Chloride 0.02 Molar is supplied in 5 ml. and 25 ml. bottles in 

 packages of 6. 



