324 DIFCO MANUAL 



BAGTO-PROTHROMBIN FREE (B445) 

 BEEF PLASMA 



Bacto-Prothrombin Free Beef Plasma is prepared according to the method of 

 Quick^'2.3 for use in the prothrombin consumption tests and for diluting human 

 plasma for prothrombin time determinations according to the method of Rosen- 

 field and Tuft.-i 



To rehydrate Bacto-Prothrombin Free Beef Plasma, add 4.5 ml. distilled water 

 and rotate gently in an end over end motion to effect solution without generating 

 excessive air bubbles. The reconstituted plasma is equivalent to freshly prepared 

 deprothrombinized beef plasma. Bacto-Prothrombin Free Beef Plasma as well as 

 the reconstituted plasma should be stored in the refrigerator at 2-6° C. 



Bacto-Prothrombin Free Beef Plasma is supplied in 5 ml. vials in packages 

 of 6. 



1 Blood, 4:1281:1949. 3 Quick, The Physiology and Pathology of 



2 Am. J. Clin. Path., 19:1016:1949. Hemostasis, 142:1951. 



* Am. J. Clin. Path., 17:405:1947. 



BAGTO-PROTHROMBIN FREE (B446) 

 RABBIT PLASMA 



Bacto-Prothrombin Free Rabbit Plasma is prepared according to the method 

 of Quicki'2.3 for use in the prothrombin consumption test. It may also be used 

 for diluting plasmas for prothrombin time determinations by the method de- 

 scribed by Rosenfield and Tuft.* 



To rehydrate Bacto-Prothrombin Free Rabbit Plasma, add 4.5 ml. distilled 

 water to each 5 ml. vial. Rotate gently in an end over end motion to effect solu^ 

 tion without generating excessive air bubbles. The reconstituted plasma is equiv- 

 alent to freshly prepared deprothrombinized rabbit plasma. Bacto-Prothrombin 

 Free Rabbit Plasma as well as the reconstituted plasma should be stored in the 

 refrigerator at 2-6° G. 



Bacto-Prothrombin Free Rabbit Plasma is supplied in 5 ml. vials in packages 

 of 6. 



^ Bloody 4j^i 281 M 949. _ 8 Quick, The Physiology and Pathology of 



: 405: 1947- 



2 Am. J. Clin. Path., 19:1016:1949. Hemostasis, 142:1951. 



*Am. J. Clin. Path., 17: 



BAGTO-GEPHALIN GHOLESTEROL (B238) 

 ANTIGEN 



Bacto-Cephalin Cholesterol Antigen is recommended for use in the Hanger 

 flocculation test^'^ for determining the index of disturbance of the liver paren- 

 chyma. It is a stable desiccation consisting of 1 part especially prepared sheep 

 brain cephalin and 3 parts Bacto-Cholesterol. 



The Hanger flocculation test is regarded as an index of inflammatory and 

 certain other forms of degenerative disturbances of the liver parenchyma, and 

 does not necessarily parallel hepatic function tests. Cephalin cholesterol emul- 

 sions, properly prepared, are not flocculated by sera from normal individuals but 

 are flocculated by sera from patients with active disturbance of the liver paren- 

 chyma. The degree of flocculation parallels the severity of active liver disease 

 and may, therefore, be employed prognostically in estimating the degree and 



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