DIAGNOSTIC REAGENTS 



337 



Antibiotic 



Aureomycii 

 Bacitracin 



in 



joacitracin 



Chloromycetin 



Dihydrostrepto- 



mycin 

 Penicillin 

 Polymyxin B 



(Aerosporin) 

 Terramycin 



Color 



Yellow 

 Brown 

 Grey 

 Pink 



Plain 



10 meg. 



2 units 



10 meg. 



1 meg. 



Marking 



Green 0.5 unit 

 Blue 5 meg. (50 



units) 

 Terra-cotta 10 meg. 



Sets are also available consisting of one vial each of the lowest (B403A), inter- 

 mediate (B403B) or highest (B403C) concentration of each therapeutic agent. 



Replacement packages consist of six vials of any one concentration of any 

 antibiotic. 



Streptomycin (white disks) in concentrations of 1.0, 10 and 100 meg. are avail- 

 able also in replacement packages. 



1 J. Path. Bact., 57 = 379: i945- 



a Am. J. Med. Tech., 12:251:1946. 



^ Am. J. Med. Sciences, 213:221:1947. 



* Approved Laboratory Technique, 5th Edi- 

 tion: 557:1 951. 



BJ. Lab. Clin. Med., 35:188:1950. 



* J. Lab. Clin. Med., 35:205:1950. 

 'Am. J. Clin. Path., 20:325:1950. 

 8 Am. J. Clin. Path., 20:335:1950. 

 8 Lancet, 2:641:1948. 



10 Laryngoscope, 60: 131 : 1950. 



"Can. Jf. Pub. Health, 43:35:1952. 



^ Navy Med. Research Inst. Research Report, 



Project NM 005 048, 04.13. 

 "News Letter S.A.B., 17:15:1951. Paper 



read at Mich. Branch S.A.B. Mtg. April, 



1951- 



1* Laryngoscope, 6 1 : 649 : 1 95 1 . 



"J. Am. Med. Assoc, 147:1336:1951. 



^8 Am. J. Clin. Path., 21:1094:1951. 



1' J. Antibiotics and Chemotherapy, 2:30:1952. 



18 J. Urol. In Press. 



18 Med. Technicians Bull., 3:7:1952. 



* Bact. Proceedings, Boston, p. 120:1952. 



21 Antibiotics and Chemotherapy, 2:229:1952. 



22 Edinburgh Med. J., 59:178:1952. 



23 Surg. Gynecol. Obstet., 9:669:1950. 

 2* Am. J. Clin. Path., 21:884:1951. 



35 J. Bact., 59:711:1951. 



^ Personal Communication. 1950. 



^Am. J. Clin. Path., 20:682:1950. 



BAGTO-GONGENTRATION DISKS, PENICILLIN (B452) 

 BAGTO-SUBTILIS SPORE SUSPENSION (B453) 



Bacto-Concentration Disks, Penicillin are used to determine the penicillin level 

 of milk, serum, plasma, urine and other body fluids. Concentrations of from 

 0.05 to 5 units penicillin per ml. may be determined by this method. The Con- 

 centration Disks contain known measured amounts of penicillin to correspond to 

 0.05, 0.1, 0.25, 0.5, 1.0, 2.5 and 5.0 units per ml. Disks containing Bacto-Penase 

 are supplied to identify the bactericidal agent as penicillin. The test employs a 

 standardized medium (Bacto-Whey Agar), a standardized inoculum (Bacto- 

 Subtilis Spore Suspension), and when the conditions of the test are adhered to, 

 accurate determinations can be made. 



Churchill and Frank^'^ used the Concentration Disks, Penicillin to detect 

 penicillin in milk. They compared the phosphatase method, growth coagula- 

 tion method and the Disk method and reported the superiority of the Disk 

 method. Kosikowsky,^ in a systematic testing of inhibitory substances in milk re- 

 ported that under optimum conditions concentrations of penicillin as low as 

 0.05 unit per ml. were detected. Detailed discussion of Bacto-Concentration 

 Disks is available upon request. 

 1 Paper read at Mich. Branch S A.B. Mtg. April 1951. 3 In Press, a J. Dairy Science, 35:533:1952- 



