340 DIFCO MANUAL 



These media are packaged in 100 ml. amounts in screw capped bottles. They 

 are especially recommended for blood culture work, each bottle containing 

 sufficient medium for up to 10 ml. of blood or other body fluid as inoculum. 



BACTO 



PENASE BROTH (B347B) 



Bacto-Penase Broth is prepared especially for use in culturing organisms from 

 blood and other body fluids from patients under penicillin, streptomycin, or 

 sulfonamide treatment. It is prepared from Bacto-Brain Heart Infusion and 

 contains sufficient Bacto-Penase and para-Aminobenzoic Acid to inactivate any 

 penicillin or sulfonamide that might be carried over in a body fluid inoculum. 

 One hundred ml. of this medium will inactivate 10,000 units of penicillin and up 

 to 1000 units of streptomycin. 



Each bottle of Bacto-Penase Broth contains 100 ml. of medium so as to 

 permit the use of as much as 10 ml. of blood or other body fluid as an inoculum. 

 Inoculation must be made under aseptic conditions. Contamination can best be 

 avoided if the cap is held in the hand, bottom down, to avoid its contact with 

 contaminated objects. After replacing the cap the bottle is gendy agitated to 

 distribute the inoculum uniformly throughout the medium. Incubate at 37 °C. 

 Observations for growth are made at desired intervals, preferably after 24 hours 

 and on each succeeding day for 14 days. Material for examination may be re- 

 moved with either a sterile pipette or loop using aseptic precautions. 



Bacto-Penase Broth is stable when stored at refrigerator temperatures. At 

 normal room temperatures, the Bacto-Penase in the medium deteriorates slowly 

 and for that reason the medium must be placed in the refrigerator immediately 

 upon receipt and kept there until used. Stability tests to date indicate that the 

 Bacto-Penase in the medium is stable for at least a year when stored in the 

 refrigerator. This medium must not be heated before inoculation as the Bacto- 

 Penase is thermolabile. 



Bacto-Penase Broth is supplied in packages of one-half dozen bottles of 100 ml. 

 each. 



BACTO-PENASE MEDIUM (B348B) 



Bacto-Penase Medium is prepared especially for use in culturing organisms 

 from blood and other body fluids from patients under penicillin, streptomycin, 

 or sulfonamide treatment. It is prepared from Bacto-Brain Heart Infusion with 

 PAB and Agar, and contains sufficient Bacto-Penase and para-Aminobenzoic 

 Acid to inactivate any penicillin or sulfonamide that might be carried over in a 

 body fluid inoculum. One hundred ml. of this medium will inactivate 10,000 

 units of penicillin and up to 1000 units of streptomycin. The presence of the 

 small amount of agar in the medium provides a range of oxidation-reduction 

 potentials, permitting the development of anaerobes and microaerophiles, as well 

 as aerobes. 



Each bottle of Bacto-Penase Medium contains 100 ml. of medium so as to 

 permit the use of as much as 10 ml. of blood or other body fluid as an inoculum. 

 Inoculation must be made under aseptic conditions. Contamination can best be 

 avoided if the cap is held in the hand, bottom down, to avoid its contact with 

 contaminated objects. After replacing the cap the bottle is gently agitated, to 

 distribute the inoculum uniformly throughout the medium. Incubate at 37°C. 



