MISCELLANEOUS PRODUCTS 341 



Observations for growth are made at desired intervals, preferably after 24 hours 

 and on each succeeding day for 14 days. 



Upon storage, and especially upon the addition of the inoculum, there may 

 be a partial flocculation of the agar in the medium. This in no way interferes 

 with the nutritional value of the medium. 



Bacto-Penase Medium is stable when stored at refrigerator temperatures. At 

 normal room temperatures, the Bacto-Penase in the medium deteriorates slowly 

 and for that reason the medium must be placed in the refrigerator immediately 

 upon receipt and kept there until used. Stability tests to date indicate that the 

 Bacto-Penase in the medium is stable for at least a year when stored in the re- 

 frigerator. This medium must not be heated before inoculation as the Bacto- 

 Penase is thermolabile. 



Bacto-Penase Medium is supplied in packages of one-half dozen bottles of 

 100 ml. each. 



BAGTO-FLUID THIOGLYGOLLATE (B256B) 

 MEDIUM 



Bacto-Fluid Thioglycollate Medium conforms to the formula specified by the 

 National Institute of Health for the sterility testing of biologicals, and for the 

 sterility testing of antibiotics according to the method of the Food and Drug 

 Administration, as well as the specifications given in the U. S. Pharmacopeia 

 and the National Formulary. Bacto-Fluid Thioglycollate Medium is especially 

 recommended for culturing organisms from inocula containing mercurial pre- 

 servatives. This medium has been used for the cultivation of anaerobes from 

 blood and other body fluids since the small concentration of agar present in the 

 medium prevents reaeration of the medium after sterilization by convection cur- 

 rents and thus maintains a low oxidation-reduction potential. Brain Heart In- 

 fusion with PAB and Agar or Bacto-Penase Medium not containing thioglycollate, 

 have proven more satisfactory for the cultivation of some anaerobes and aerobes 

 from materials not containing mercurials. 



Each bottle of Bacto-Fluid Thioglycollate Medium contains 100 ml. of medium 

 so as to permit the use of as much as 10 ml. of blood or other body fluid 

 as an inoculum. To test sterility use 1-2 ml. of the solution under assay. Inocula- 

 tion must be made under aseptic conditions. Contamination can best be avoided 

 if the cap is held in the hand, bottom down, to avoid its contact with con- 

 taminated objects. After replacing the cap the bottle is gently agitated to distrib- 

 ute the inoculum uniformly throughout the medium and then incubated at 

 37 °C. Observations for growth are made at desired intervals, preferably after 

 24 hours and on each succeeding day for 14 days. 



Upon storage, and especially upon the addition of the inoculum, there may be 

 a partial flocculation of the agar in the medium. This in no way interferes with 

 the nutritional value of the medium. 



Just prior to inoculation the bottles of the medium should be heated in a 

 boiling water bath or flowing steam at 100°C. for 10 minutes to drive out dis- 

 solved gases, and then cooled to below 40° C. without excessive agitation. 



Bacto-Fluid Thioglycollate Medium is supplied in packages of one-half dozen 

 bottles of 100 ml. each. 



