SYNCHRONOUS GROWTH FOR SPORE PRODUCTION 17 



but I think that this does offer some hope of cleaning up spore suspen- 

 sions. The enzyme is very like lysozyme in its activity. This is a study we 

 do intend to get on with. We are now making the enzyme in a concen- 

 trated form and are studying its properties. The next thing we mean to do 

 is to see if it will help in this dreadful problem of getting rid of these hor- 

 rible vegetative cells. 



Kr.\sk: I would like to mention a technique we have developed with 

 lysozyme that may or may not be applicable to some of this work. We find 

 that some organisms not susceptible to lysozyme at neutral pH become sus- 

 ceptible in the presence of versine and tris buffer. 



Harrell: I wonder if Mr. Collier would like to comment on the prob- 

 lem of getting spores in large volumes of media. When we get the volume 

 of medium over two liters, we get poor results. We get good vegetative 

 growth but few spores. If we keep the volume of the medium less than 1500 

 ml. we get good sporulation. 



Collier: We have had the same experience. We believe that the pH of 

 the medium may be a factor and therefore we want to repeat some of these 

 experiments with tris buffer. The medium appears to become more acid in 

 large than in small volumes. 



