NUTRITIONAL AND ENVIRONMENTAL CONDITIONS 25 



higher carbon dioxide concentration favors the production of dipicolinic 

 acid, a major and specific constituent of spores. 



We have obtained somewhat similar results (Amaha, Ordal, and Touba, 

 1956) when B. coagulans was cultured on thermoacidurans broth in 

 shake culture. Essentially no spores were produced in th^ basal medium. 

 The addition of malate, succinate, or a-keto-glutarate markedly stimulated 

 sporogenesis. but the other compounds tested (citrate, fuipsrate, oxalace- 

 tate. pyruvate, or D-alanine) were without apparent effect. . • 



In the time allotted for these remarks, I have had to leave out some per- 

 tinent data. I trust that these omissions wnll be adequately taken care of 

 in the discussion to follow. 



References 



Amaha, M.. Z. J. Ordal. and A. Touba. 1956. Sporulation requirements of 

 Bacillus coagulans var. thermoacidurans in complex media. J. Bact.. 72: 

 34-41. 



Behring, E. 1889. Beitrage zur Aetiologie des Milzbrandes. Z. Hyg. Infek- 

 tionskrankh. 6: 117-144. (Cited by Wynne, 1952). 



Behring, E. 1889. Beitrage zur Aetiologie des Milzbrandes. VI. Ueber as- 

 porogenen Milzbrand. Z. Hvg. Infektionskrankh. 7: 171-185 (Cited bv 

 Wynne, 19.52). 



Blair. E. B. 1950. Observations on Bacterial Spore Formation in Synthetic 

 Media. M. A. Thesis, Universitv of Texas. Abstracted in Texas Repts. 

 Biol. Med. 8: 361. 



Campbell. L. L. Jr. and 0. B. Williams. 1953. The effects of temperature 

 on the nutritional requirements of facultative and obligate thermophilic 

 bacteria. J. Bact. 65: 141-146. 



Foster, J. W. and F. Heiligman. 1949a. Mineral deficiencies in complex or- 

 ganic media as limiting factors in the sporulation of aerobic bacilli. 

 J. Bact. 57: 613-615. 



Foster, J. W. and F. Heiligman. 1949b. Biochemical factors influencing 

 sporulation in a strain of Bacillus cereus. J. Bact. 57: 639-646. 



Grelet. N. 1951. Le determinisme de la sporulation de B. megaterium. I. 

 L'effet de I'epuisement de I'aliment carbone en milieu svnthetique. Ann. 

 Inst. Pasteur. 81: 430-435. 



Grelet, N. 1955. Nutrition azotee et sporulation de Bacillus cereus var. 

 mycoides. Ann. Inst. Pasteur. 88: 60-75. 



Hardwick, W. A. and J. W. Foster. 1952. On the nature of sporogenesis in 

 some aerobic bacteria. J. Gen. Physiol. 35: 907-927. 



Knavsi. G. 1948. The endospore of bacteria. Bact. Rev. 12: 19-77. 



Krask, B. J. 1953 Methionine sulfoxide and specific inhibition of sporula- 

 tion in Bacillus subtilis. J. Bact. 66: 374. 



Powell, J. F. 1951. Sporulation and germination of a strain of Bacillus 

 megatherium. J. Gen. Microbiol. 5: 993-1000. 



Powell J. F. and J. R. Hunter. 1955. The sporulation of Bacillus sphaericus 

 stimulated bv association with other bacteria: an effect of carbon diox- 

 ide. J. Gen. Microbiol. 13: 54-58. 



