58 



CLARENCE F. SCHMIDT 



Table II 

 Chemical activation of spore suspension 5230-5 



Medium 



2-3 



Mean Spore Count per Plate 



Incubation Time in Days 



5-6 9-10 15-17 20-23 



* mcg/ml 



experiments carried out at different times. The numbers following the sub- 

 stances represent micrograms per ml concentration. Yeast extract at 500 or 

 50 meg activates about the same number of spores as the complex medium, 

 10 meg is somewhat less effective. One meg was without effect. Confirming 

 the results obtained by other investigators with other procedures, it was 

 found that L-alanine is an effective activator at 500 and 5 meg concentra- 

 tion. With this suspension, adenosine at 10 meg was ineffective. 



Table III shows a sumary of heat and chemical activation of the two 

 spore suspensions. In general, the behavior of the 5230-24 suspension was 

 the same as the 5230-5, the main difference being that the 5230-24 suspen- 

 sion showed somewhat more rapid germination on the basal medium and a 

 somewhat greater sensitivity to chemical activators. This table shows again 



Table III 



Heat and chemical activation of spore suspensions 



5230-5 and 5230-24 



Medium 



Percentage of Maximum Count 

 5230-5 5230-24 



DT+ST 

 DT+ST H* 



5504 



5504 H* 



5504 + Yeast extract 500' 



5504 + L-alanine 500 



* Heat activated spores 

 * * meg /mj 



