60 CLARENCE F. SCHMIDT 



Table VI 



Test for activation by substances other than 



amino acids. Suspension 5230-5 



Substance Added* Mean Spore Count per Plate 



Incubation Time in Days 

 2-3 5-6 9-10 15-17 20-23 



None 



NH4CI 1000 

 Na Pyruvate 100 

 Furfural 10 

 Mn 100 



* mcg/ml 



what the effect of L-valine, although this is eventually overcome, /^-alanine 

 also is an effective chemical activator although the rate of development of 

 colonies is somewhat slower than with L-alanine or L-valine. 



Table V shows the results of tests with several other L-amino acids. L- 

 methionine and L-serine seem to be slightly active following a long lag 

 period. L-threonine is inactive and L-leucine and L-arginine are very ques- 

 tionable as to activity. 



Table VI shows tests for activation by some substances other than amino 

 acids. NH4CI and Na pyruvate appear to show slight activity after a long 

 lag. Furfural and Mn+ ^ are inactive in the concentrations tested and, in 

 fact, in comparison to the controls, appear to be somewhat inhibitory of the 

 spontaneous germination. 



Table VII shows a summary of activation results for both suspensions 



Table VII 

 Activation by amino acids and other substances 



Strong Weak with Inactive 

 Long Lag 



L-alanine L-arginine DL-methionine 



^-alanine L-leucine DL-phenylalanine 



L-valine L-methionine L-phenylalanine 



L-serine L-glutamic acid 



NH4CI DL-serine 



Na pyruvate L-threonine 



Furfural 

 Mn 



