86 



C. F, ROBINOW 



2000 



1500 



O 1000 



500 



200 



100 







10 20 30 40 60 

 TIME (MIN) AFTER INOCULATION 



Fig. 1. The phosphorus fractions of B. cereus germinating in semi-syn- 

 thetic medium. P as mg/100 g inoculated spores. 



■, P uptake 

 D, Acid-Soluble P. 



O, RNA-P (method of Schneider, 1945). 

 • , RNA-P (method of Schmidt & Thannhauser, 1945). 



A, DNA-P (method of Schneider, 1945) 



A, DNA-P (method of Schmidt & Thannhauser, 1945). 



the rapid and early rise of ribosenucleic acid phosphorus (RNA-P) which 

 occurs following the initial awakening of the resting spores. In this par- 

 ticular study the spores iB. cereus) began to lose their refractility 2l^ 

 minutes after inoculation and were 90% non-refractile by 6 minutes. The 

 medium here was sufficient to insure rapid growth into vegetative forms. 

 In other studies, medium containing only alanine, adenosine and glucose 



