ENZYMES DORMANT IN THE INTACT SPORE 107 



Table I 



Effect of duration of heat shock and concentration of nutrients on 



oxidation of glucose by spores of B. terminalis 



Strength of spore suspension — 6 mg dry wt/ml 



Substrate — Glucose 0.5 mg/ml ix 1 oxygen uptake in 30 min. 



suspension. A dilute spore suspension (1 mg dry wt/ml) required a mini- 

 mum of 9 hours, but even then the amount of glucose oxidizing enzymes 

 activated, as judged by the difference between the exogenous and endogenous, 

 was very low. Furthermore, the endogenous oxidation decreased with in- 

 creasing strength of the spore suspension and duration of heat shock (Table 

 II). 



L-alanine alone with heat was effective in activating the enzymes. Heat 

 shock with adenosine alone was ineffective. Heat shock with L-alanine and 

 adenosine together was no more effective than with L-alanine alone. However, 



Table II 



Effect of duration of heat shock and strength of spore suspension 



on oxidation of glucose by spores of B. terminalis 



Nutrients — 26 /xg/ml L-alanine, 6 ju,g/ml adenosine 

 Substrate— Glucose 0.5 m/ml /i 1 oxygen uptake in 30 



min. 



