108 G. G. KRISHNA MURTY 



Table III 



Eflfect of time of addition of nutrients on oxidation of glucose 



by heat shocked spores of B. terminalis 



Strength of spore suspension — 15 mg dry wt/ml 



Substrate — Glucose 0.5 mg/ml 



Duration of heat shock — 5 hrs. at 65°C fx I oxygen uptake in 30 minutes 



the L-alanine had to be added before heat shock. If L-alanine or adenosine 

 was added after heat shock, the spores germinated partially and, as a con- 

 sequence, both the endogenous and exogenous oxidation were very high 

 (Table III). 



The enzymes are not active immediately after heat shock, but only after 

 40-45 min. incubation at 30 °C following the heat shock. However, the L- 

 alanine could be washed out immediately after heat shock or later without 

 any significant change in activity. In contrast, germinated spores lost nearly 

 all their glucose-oxidizing activity on washing (Table IV). 



P-fluorophenylalanine prevented the heat activation of these enzymes when 

 added along with L-alanine before heat shock, but had no effect when added 

 after heat shock. Furthermore, p-fluorophenylalanine had no effect on germi- 

 nation of the spores in the presence of the normal quantities of the nutrients 

 (Table V). 



When a relatively dilute, activated suspension of spores (6 mg/ml) was 



Table IV 



Eflfect of washing on oxidation of glucose by heat shocked 



spores of B. terminalis 



Time of washing Endogenous Exogenous 



No washing 4 66 



Immediately after heat shock 6 70 



40 minutes after heat shock 8 60 



Germinated spores lose nearly all glucose-oxidizing activity on washing. 



Duration of heat shock — 5 hrs. at 65 ''C 



Strength of spore suspension — 15 mg dry wt/ml 



Nutrients — 26 /^g/ml L-alanine; 6 /xg/ml adenosine 



Substrate — Glucose 0.5 mg/ml fx 1 oxygen uptake in 30 minutes 



