NO.\-0\IDATIVE KNZYMKS OF SI'OKK EXTKACTS 



131 



0.01 0.05 0.1 0.5 1.0 



MANGANESE CONC'N-MILLIMOLAR 



Fig. 10. The effect of manganous sulfate on pyrophosphatase activity of 

 B. megaterium spore extract. Reaction systems contained 0.25 ml. of spore 

 extract prepared in Mickle Disintegrator (25 mg spores per ml extract); 

 0.05 ml veronal buffer. 0.05M; 0.25 ml NaiP-Or (final concentration. 

 O.OOIM) ; and 1.0 ml of manganous sulfate. Reaction stopped by addition 

 of 0.2 ml 100'^ tricholoroacetic acid after incubation for 30 min. at 37°C. 



Fig. 11. pH-activity cur^e for spore extract pyrophosphatase. Conditions 

 as for Fig. 10, except that acetate buffer (0.05M) was used at pH 1^ 5. 

 and 6. Divalent metal ions, as sulfates, in a final concentration of 5 x 

 10-4 M. 



