148 



HARLYN HALVORSON 



^0 50 100 150 



Minutes 



Fig. 5. Pyruvate formation during glucose oxidation by spore extracts. 

 See legend Fig. 3 for details. Flask contained 5 x 10 '* M DPN. After depro- 

 teinization glucose was determined by a reducing method and pyruvate by 

 the Friedman and Haugen ( 1942 ) procedure. 



vate by either dehydration to 2-keto-3-desoxy-C-phosphogluconate ( 2KDPG) 

 and cleavage to pyruvate and D-glyceraldehyde-3-phosphate, or by oxidation 

 to a mixture of ribose-5-phosphate and ribulose-5-phosphate, which are then 

 converted to sedoheptulose-7-phosphate. F-6-P. G-6-P and D-glyceraldehyde- 

 3-phosphate. The second alternate pathway involves a direct oxidation of 

 glucose to gluconate prior to phosphorylation. Gluconate is oxidized to 2KG 

 and phosphorylated to 2K6PG which by an undefined pathway is converted 

 to 2 moles of pyruvate. 



Absence of an Embden-Meyerhofj glycolytic system 



The absence of a functional glycolytic system in the activated spore, or 

 in spore extracts, seems evident from the following observations. Activated 

 spores or spore extracts were incapable of fermenting glucose, G-l-P, F-l-P, 

 or HDP. Furthermore, the oxidation of glucose by transclucent spores was 

 resistant to NaF, a normal inhibitor of glycolysis ( Hatchisuka et al, 1956). 

 An analysis of the end products of glucose oxidation by spore extracts failed 



