OXIDATIVE ENZYMES OF BACTERIAL SPORE EXTRACTS 



151 



10 20 30 10 20 30 



SECONDS SECONDS 



Fig. 8. Reduction of DPN and TPN by spore extracts. The Beckman cu- 

 vets contained 1 ml of M glycylglycine buffer, pH 7.4, 6.7 x lO'^ M DPN 

 or TPN, 1,67x10"^ M substrates, 1 ml of dialyzed spore extract and distilled 

 water to make a final volume of 3.0 ml. The temperature was 25C. The 

 reduction was measured at 340 mix. 



Table 1 



Distribution of glucose dehydrogenase after sonic rupture 



of the spore 



Sample 



Dehydrogenase^ Nitrogen" 



Specific 

 activity 



Original extract 

 Supernate 140,000 xg 

 Residue 140,000 xg 



1 ul O.Vml/hr 

 2mgN/nil 



Warburg flask contents : side arm, 1 mg glucose and 5 x 10"^ M DPN : 

 center well; 0.2 ml 30 per cent KOH: main compartment; 1 ml of enzyme 

 preparation and sufficient 0.067 M phosphate buffer, pH 7.2, to bring the 

 flask contents to 2.0 ml. Reaction was run at 30C under atmospheric oxygen. 



