Appendix _195_- Media for Study 



12. Egg albiiiiioi agar: 



Glucose 10 gm 



K.HPO, 0.5 gm 



MoSO,.7H.O 0.2 om 



Fe^CSO.).-. Trace 



Egg albumen 0.15 om 



Agar 1 5 gm 



Distilled water 1000 ml 



Egg albumen is first dissolved in water and made neutral 

 "to phenolphthalein with N/10 NaOH. 



13. Potato-nutrient agar: 



Peeled potatoes 500 gm 



Peptone 10 gm 



Meat extract 10 gm 



NaCl 5 gm 



Agar 1 5 gm 



Tap water 1000 ml 



pH 7.0 



The potatoes are cut into small cubes to which 350 ml 

 of water is added and the whole steamed for three- 

 quarters of an hour. The extract is strained through 

 fine muslin without squeezing the pulp. The other 

 nutrients are dissolved in 350 ml of water which is 

 then added to the potato extract, and the whole steamed 

 for three-quarters of an hour. The mixture is then 

 made up to bulk, standardized and filtered, after which 

 the agar is added. 



14. Potato-glucose agar: 



Peeled potatoes 300 gm 



Glucose 5 gm 



Agar 20 gm 



Tap water 1000 ml 

 2^H 6.8 



15. Starch nitrate agar: 



Formula same as in medium 19, with addition of 1.5 per 

 cent agar. 



16. Glucose broth: 



Glucose 10 gm 



Peptone 5 gm 



Meat extract 5 gm 



NaCl 5 gm 



Distilled water 1000 ml 

 pH 7.1 



Frequently tap water is used, as in media for the produc- 

 tion of streptomycin. 



17. Nutrient hroth: 



As above, but free from glucose. 



18. Sticrose solution: 



Same as for No. 1, free from agar. 



