Waksman 



196 



Actinomycetes 



19. Starch solution: 



Soluble starch 



K,HP04 



MgSO,.7H.O 



KCl 



NaNOs 



CaCOs 



Distilled water 



Starch is made 



into a paste and boili 



20 gm 



Igm 



0.5 gm 



0.5 gm 



2gm 



2gm 

 1000 ml 

 2 water added. 



Mixture steamed for an hour to give a clear solution. 



20. Yeast extract medium A: 



Autolyzed yeast extract 



Glucose 



Distilled water 



pH 6.0 to 7.0 (383) 



21. Yeast extract medium B: 



Yeast extract 



Glucose 



NaCl 



MgS04.7H.O 



FeS04.7H.O 



Distilled water 



22. Yeast extract agar: 



Yeast extract 

 Glucose, technical 

 Agar 



Tap water 

 pH 6.8 



23. Emerson's medium: 



Beef extract 



Peptone 



NaCl 



Yeast extract 



Glucose 



Distilled water 



2.5 gm 

 5gm 

 1000 ml 



4.0 gm 

 4.0 gm 

 2.5 gm 

 1.0 gm 

 10.0 gm 

 1000 ml 



24. Com steep medium: 

 Peptone 

 Corn steep 

 NaCl 

 Glucose 

 Distilled water 



5.0 gm 

 15.0 gm 



5.0 gm 

 10.0 gm 

 1000 ml 



25. Soybean medium: 



Soybean meal 



Commercial glucose 



NaCl 



Curbay B.G. 



CaCO,, 



Distilled water 



10.0 gm 



10.0 gm 



5.0 gm 



0.5 gm 



1.0 gm 



1000 ml 



