32 ACTINOMYCETES 



a 1 . Cells acid-fast. 



Nocardia farcinica 

 b 1 . Cells non-acid-fast. 



Nocardia putoriae 

 IV. Cultures pigmented green. 



1. Saprophytes. . .Nocardia viridis 



2. Parasites Nocardia pyogenes 



V. Cultures black. 



1. Saprophytes. . .Nocardia niger 



2. Parasites Nocardia sendaiensis 



Classification System of Waksman and Henrici 



A. Partially acid-fast organisms with strongly refractive cells; nonproteo- 

 lytic and generally nondiastatic ; capable of utilizing paraffin. 

 I. Initial mycelium fully developed, well-branching, dividing into rods 

 and generally into cocci. 



1. Vegetative mycelium soft, without macroscopically visible aerial 

 mycelium. 



a. Vegetative mycelium yellow, orange, or red. 

 a 1 . Pathogenic. 



a 2 . Vegetative mycelium white, buff, or pale yellow. 



1. Nocardia farcinica 



b 2 . Vegetative mycelium yellow to red. 



2. Nocardia asteroides 

 b 1 . Not pathogenic. 



3. Nocardia polychromogenes 



b. Vegetative mycelium white to pink. 

 a 1 . Gelatin not liquefied. 



a 2 . Growth on nutrient agar opaque, cream-colored; coc- 

 coid forms in broth. 



4. Nocardia opaca 



b 2 . Growth on nutrient agar watery; no coccoid forms in 

 broth. 



5. Nocardia erythropolis 

 c 2 . Growth on nutrient agar pink. 



a 3 . Aerial mycelium on milk white. 



6. Nocardia leishmanii 

 b 3 . Pellicle on milk pink. 



7. Nocardia caprae 

 c 3 . Pellicle on milk yellow. 



8. Nocardia pretoriana 



