40 ACTINOMYCETES 



4. Streptomyces annulatus (Beijerinck emend. Krassilnikov) comb, 

 nov. (Krassilnikov, N. A., Actinomycetales, Akad, Nauk, USSR, Moskau, 

 1941, 40.) 



Aerial mycelium: Whitish or light gray. Sporophores produce spirals, 

 with 3 to 7 turns (sinistrorse) ; spores spherical, 0.7 fi. 



Synthetic agar: Growth colorless, flat, penetrating deep into agar. Aerial 

 mycelium white, velvety, growing in the form of concentric rings. 



Nutrient agar: Aerial mycelium white, concentric rings less marked. 



Gelatin: Liquefaction slow. 



Milk: Coagulation, slow peptonization. 



Starch : Hydrolysis. 



Cellulose: Growth good. 



Sucrose: Inversion. 



Odor: Strong, earthy. 



Antagonistic properties: Highly antagonistic against mycobacteria and 

 gram-positive bacteria; some strains are active against fungi. 



5. Streptomyces rochei Berger, Jampolsky, and Goldberg. (Berger, J., 

 et al., Arch Biochem., 22, 1949, 476^78.) 



Vegetative growth: On synthetic (calcium malate) and nonsynthetic 

 (peptone-tomato paste) agars, vegetative mycelium is characteristically 

 fine, 0.8 to 1.5 n in diameter, with short branches. 



Aerial mycelium: Sporogenous hyphae 1.5 n in diameter, often, but not 

 always, spirally twisted; spirals usually short and loose, with rarely more 

 than 2 to 3 coils. Spores oval to elliptical, sometimes spherical, 1.3-2.8 by 

 0.8-1.5 n. 



Synthetic agar: Growth thin, colorless, covered with sandy lavender to 

 dark gray aerial mycelium. Reverse of growth light gray, later becoming 

 grayish yellow. No soluble pigment. 



Nutrient agar: Growth cream-colored. Aerial mycelium white. No soluble 

 pigment. 



Glucose agar: Growth smooth, yellowish, covered with white to gray 

 aerial mycelium. Yellowish soluble pigment. 



Potato: Growth abundant, lichnoid, cream-colored. Aerial mycelium 

 abundant, cottony, white to gray. Color of plug becomes reddish tan. 



Gelatin (18°C): Surface cream-colored ring, covered with white aerial 

 mycelium. Liquefaction rapid. Faint yellow soluble pigment. 



Milk: Ring at surface cream-colored to brownish. Coagulation and rapid 

 peptonization. 



Starch: Growth brownish. Aerial mycelium mouse-gray. Reverse shows 

 slight purple pigmentation in 4 days. Diastatic action strong. 



Calcium malate-glycerin: Growth good, raised in center. Aerial mycelium 



