SPECIES OF STREPTOMYCES 41 



gray, buff around the edges, having a fuzzy appearance. Medium is cleared 

 directly under the growth. 



Calcium citrate-glycerol broth : Growth at surface forming a thin mat of 

 partially sporulated, discrete, grayish white colonies. From the mat, soft, 

 round, fuzzy gray colonies drop into the medium. 



Antagonistic properties: On certain complex nitrogenous media, such as 

 those containing soybean flour or distillers' dried solubles, the organism 

 produces a wide range of antimicrobial activity. Part of this is attributable 

 to a specific antibiotic known as borrelidin. 



Remarks: Morphologically the culture resembles some species in the 

 Streptomyces albus group, such as Streptomyces albidoflavus, Streptomyces 

 calif or nicus, Streptomyces lipmanii, but it is not believed to be identical 

 with any of them. 



6. Streptomyces coelicolor (Muller) Waksman and Henrici. (Miiller, 

 R., Centrbl. Bakt. I, 46, 1908, 195). 



Aerial mycelium: Straight filaments with open, dextrorse spirals. Spores 

 oval or rod-shaped, 0.7-1.0 by 0.8-1.5 y.. 



Synthetic agar: Growth thin, spreading, colorless at first, becoming red, 

 then blue. Aerial mycelium thin, powdery, white, becoming mouse-gray. 



Nutrient agar: Growth good. Pigment lacking or faint blue. 



Potato: Strong pigment production, sometimes greenish blue or violet, 

 but usually sky-blue, diffusing through medium and coloring water at base 

 of tube. 



Gelatin: Growth good. No soluble pigment. Liquefaction rapid. 



Milk: No change at 25°C. At 37°C, coagulation, with rapid peptonization. 



Nutrient broth: Growth good. Cretaceous layer around edge. 



Nitrate: Reduction positive. 



Aspargine agar: Growth with glycerol as source of carbon, good violet 

 to deep blue, with pigment diffusing through medium; final pH 7.0-8.0. 

 Growth with glucose as source of carbon, poorer, red, no diffusion of pig- 

 ment; final pH 6.0 to 5.0. 



Blood agar: Hemolysis on 4th day. 



Temperature: Growth good at room temperature and at 37°C. 



Antagonistic properties: Some strains produce coelicolorin and mycetin. 



Remarks: Muller reported no acid from carbohydrates on organic media. 

 Conn recorded acid from glucose and lactose, and sometimes from sucrose 

 and mannitol, on synthetic media. 



The most striking characteristic of this organism is a litmus-like pigment, 

 usually produced on potato or synthetic media, which is deep blue and 

 water-soluble at alkaline reactions, violet around neutrality, and red (in- 

 soluble in water) at about pH 6.0. The primary pigment has a spectro- 



