50 ACTINOMYCETES 



23. Streptomyces lieskei (Duch6) Waksman and Henrici. (Duch6, 

 J., Les actinomyces du groupe albus, P. Lechevalier, Paris, 1934.) 



Synthetic agar: Growth cream-colored with delayed white aerial myce- 

 lium growing from the edge toward the center; mycelium later yellowish. 

 Reverse of growth yellowish to green. Dirty yellow to yellow-green soluble 

 pigment. 



Nutrient agar: Growth cream-colored becoming covered with white 

 aerial mycelium; yellowish soluble pigment. 



Gelatin: Growth cream-colored becoming covered with white aerial my- 

 celium; no soluble pigment. Rapid liquefaction. 



Synthetic solution: Long branching filaments, 0.7 n in diameter. Aerial 

 mycelium yellowish white; does not readily produce spores; flakes drop to 

 the bottom of the tube. 



Peptone solution: Cream-colored colonies on surface with flakes in the 

 liquid dropping to the bottom of the tube. Liquid becomes yellowish. 



Tyrosine medium: Rapid growth on surface with whitish yellow aerial 

 mycelium; yellowish to orange-yellow soluble pigment. 



Milk: Growth cream-colored; colorless on reverse side; no aerial myce- 

 lium. Peptonization without coagulation. After 20 days the whole milk be- 

 comes a clear yellowish liquid. 



Coagulated serum: Growth clear-colored. Liquefaction rapid. 



Remarks: Culture related to Streptomyces alboflavus and Streptomyces 

 albidoflavus. 



24. Streptomyces flavovirens (Waksman) Waksman and Henrici. 

 (Waksman, S. A., Soil Sci., 8, 1919, 117.) 



Aerial mycelium: Large masses of minute tufts; the hyphae coarse, 

 straight, short, relatively unbranched, beaded; open spirals may be pro- 

 duced in certain substrates. Conidia spherical, oval to rod-shaped, 0.75- 

 1.0 by 1.0-1.5 ji. 



Synthetic agar: Growth spreading deep into the substratum, yellowish 

 with greenish tinge. Aerial mycelium gray, powdery. 



Nutrient agar : Growth yellowish ; the reverse dark in center with yellow- 

 ish zone and outer white zone. 



Glucose agar: Growth restricted, developing only to a very small extent 

 into the medium, yellow, turning black, edge entire. 



Potato: Growth sulfur-yellow, wrinkled. 



Gelatin: Yellowish green surface pellicle, consisting of a mass of small 

 colonies, on the liquefied medium. 



Milk: Cream-colored to brownish ring; coagulation; peptonization, be- 

 coming faintly alkaline. 



