SPECIES OF STREPTOMYCES 51 



Starch: Growth greenish yellow, spreading, developing deep into the 

 medium. Hydrolysis. 

 Glucose broth: Thick, sulfur-yellow pellicle or ring. 

 Nitrate: Reduction to nitrite very slight. 

 Temperature: Optimum 25°C. 

 Antagonistic properties: Active against fungi. 

 Remarks: Greenish yellow soluble pigment formed. 



24a. Streptomyces celluloflavus Nishimura, Kimura, and Kuroya 

 (Nishimura, H., Kimura, T., and Kuroya, M., J. Antibiotics [Japan], 6, 

 1953, 57-65.) 



Aerial mycelium: A few imperfect spirals. Almost spherical spores, 

 1.0 by 0.9 m. 



Synthetic agar: Glossy growth, developing deep into medium, later 

 becoming marguerite-yellow color. Faint sulfur-yellow soluble pigment. 



Calcium malate glycerol agar: Primrose-yellow growth, later turning 

 white to pale olive-buff, with blackish center. Cottony aerial mycelium 

 white, with grayish patches, later turning olive-buff. Citron-yellow soluble 

 pigment. 



Glucose agar: Cream to yellow-colored growth. Scant cottony white to 

 gray aerial mycelium. Sulfur-yellow soluble pigment. 



Nutrient agar: Olive-buff, turning colorless, growth. Scant, cottony, 

 white to grayish aerial mycelium. Yellow with tinge of green, to old-gold 

 soluble pigment. 



Potato: Wrinkled, spreading, deep-olive-buff colored growth. White to 

 olive-buff aerial mycelium. Deep olive-buff soluble pigment. 



Gelatin: Ivory-yellow to olive-buff colonies on surface of liquefied portion. 

 No aerial mycelium. Faint brownish pigment. Rapid to medium liquefac- 

 tion. 



Milk: Yellow to dark olive-buff growth. White aerial mycelium. Reddish 

 brown soluble pigment. Coagulation and rapid peptonization. 



Tyrosine medium: Ivory yellow to cream-buff growth. None to scant 

 white aerial mycelium. Greenish yellow soluble pigment. 



Cellulose agar: Poor growth, with yellow soluble pigment. 



Antagonistic properties: Produces thiolutin. 



25. Streptomyces limosus Lindenbein (Lindenbein, W., Arch. Mikro- 

 biol., 17, 1952, 361-383.) 



Vegetative growth: Crumb-like, yellowish, later becoming gray to coal- 

 black; greenish yellow reverse. 



Aerial mycelium: Powdery snow-white to gray, or completely lacking. 

 Soluble pigment greenish yellow to citron-yellow. 



